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Paper Information

Journal:   IRANIAN BIOMEDICAL JOURNAL   JULY 2016 , Volume 20 , Number 3; Page(s) 182 To 186.
 
Paper: 

A MODEL TO STUDY THE PHENOTYPIC CHANGES OF INSECT CELL TRANSFECTION BY COPEPOD SUPER GREEN FLUORESCENT PROTEIN (COP-GFP) IN BACULOVIRUS EXPRESSION SYSTEM

 
DOI: 

10.7508/ibj.2016.03.008

 
Author(s):  SHOKROLLAHI NARJES, SHAHBAZZADEH DELAVAR, POOSHANG BAGHERI KAMRAN, HABIBI ANBOUHI MAHDI, JAHANIAN NAJAFABADI ALI, BEHDANI MAHDI*
 
* BIOTECHNOLOGY RESEARCH CENTER, VENOM AND BIOTHERAPEUTICS MOLECULES LAB, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 

Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells.
Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells.
Results: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.
Conclusion: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.

 
Keyword(s): BACULOVIRUS, SF9, TRANSFECTION
 
 
References: 
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Citations: 
 
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APA: Copy

SHOKROLLAHI, N., & SHAHBAZZADEH, D., & POOSHANG BAGHERI, K., & HABIBI ANBOUHI, M., & JAHANIAN NAJAFABADI, A., & BEHDANI, M. (2016). A MODEL TO STUDY THE PHENOTYPIC CHANGES OF INSECT CELL TRANSFECTION BY COPEPOD SUPER GREEN FLUORESCENT PROTEIN (COP-GFP) IN BACULOVIRUS EXPRESSION SYSTEM. IRANIAN BIOMEDICAL JOURNAL, 20(3), 182-186. https://www.sid.ir/en/journal/ViewPaper.aspx?id=511674



Vancouver: Copy

SHOKROLLAHI NARJES, SHAHBAZZADEH DELAVAR, POOSHANG BAGHERI KAMRAN, HABIBI ANBOUHI MAHDI, JAHANIAN NAJAFABADI ALI, BEHDANI MAHDI. A MODEL TO STUDY THE PHENOTYPIC CHANGES OF INSECT CELL TRANSFECTION BY COPEPOD SUPER GREEN FLUORESCENT PROTEIN (COP-GFP) IN BACULOVIRUS EXPRESSION SYSTEM. IRANIAN BIOMEDICAL JOURNAL. 2016 [cited 2021July28];20(3):182-186. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511674



IEEE: Copy

SHOKROLLAHI, N., SHAHBAZZADEH, D., POOSHANG BAGHERI, K., HABIBI ANBOUHI, M., JAHANIAN NAJAFABADI, A., BEHDANI, M., 2016. A MODEL TO STUDY THE PHENOTYPIC CHANGES OF INSECT CELL TRANSFECTION BY COPEPOD SUPER GREEN FLUORESCENT PROTEIN (COP-GFP) IN BACULOVIRUS EXPRESSION SYSTEM. IRANIAN BIOMEDICAL JOURNAL, [online] 20(3), pp.182-186. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511674.



 
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