Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN BIOMEDICAL JOURNAL   JULY 2016 , Volume 20 , Number 3; Page(s) 175 To 181.
 
Paper: 

COMPARISON OF BIOFILM FORMATION BETWEEN METHICILLIN-RESISTANT AND METHICILLIN-SUSCEPTIBLE ISOLATES OF STAPHYLOCOCCUS AUREUS

 
DOI: 

10.7508/ibj.2016.03.007

 
Author(s):  GHASEMIAN ABDOLMAJID, NAJAR PEERAYEH SHAHIN*, BAKHSHI BITA, MIRZAEE MOHSEN
 
* DEPT. OF BACTERIOLOGY, FACULTY OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background: The aim of this study was to compare the biofilm formation and the prevalence of biofilm-associated genes between the isolates of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus.
Methods: In total, 209 S. aureus isolates were collected. The antibiotic susceptibility test was conducted using nine antibiotics according to the guidelines of Clinical and Laboratory Standards Institute. Phenotypic biofilm formation was performed with microtiter plate assay. The polymerase chain reaction was employed to detect icaA, icaD, icaB, icaC, clfA, clfB, fnbA, fnbB, fib, cna, eno, ebps, bbp, mecA, and SCCmec types as well as agr group genes with specific primers.
Results: Sixty-four (30.62%) isolates were resistant to methicillin, and 54 (83%) MRSA harbored SCCmec III. Furthermore, 122 (58.3%) isolates belonged to agr group I. Twenty-six (36.1%) MRSA and 42 (28.9%) MSSA isolates were strong biofilm producers (no significant difference). The prevalence of icaA, icaD, icaB, and icaC genes in MSSA isolates was 71, 41, 76, and 72%, respectively. The frequency of clfA, clfB, fnbA, fnbB, fib, cna, eno, ebps, and bbp in MSSA was 100, 100, 56, 46, 74, 54, 78, 11, and 1%, respectively. However, in MRSA isolates, the frequency was 97, 97, 64, 51, 76, 56, 79, and 12% with no track of bbp, respectively.
Conclusion: Statistical difference between MSSA and MRSA regarding biofilm formation and the frequency of all biofilm-encoding genes was not significant. The majority of the S. aureus isolates harbored clfA, clfB, eno, fib, icaA, and icaD genes.

 
Keyword(s): BIOFILM, METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS, METHICILLIN-SUSCEPTIBLE STAPHYLOCOCCUS AUREUS
 
 
References: 
  • Not Registered.
  •  
  •  
 
Citations: 
 
+ Click to Cite.
APA: Copy

GHASEMIAN, A., & NAJAR PEERAYEH, S., & BAKHSHI, B., & MIRZAEE, M. (2016). COMPARISON OF BIOFILM FORMATION BETWEEN METHICILLIN-RESISTANT AND METHICILLIN-SUSCEPTIBLE ISOLATES OF STAPHYLOCOCCUS AUREUS. IRANIAN BIOMEDICAL JOURNAL, 20(3), 175-181. https://www.sid.ir/en/journal/ViewPaper.aspx?id=511666



Vancouver: Copy

GHASEMIAN ABDOLMAJID, NAJAR PEERAYEH SHAHIN, BAKHSHI BITA, MIRZAEE MOHSEN. COMPARISON OF BIOFILM FORMATION BETWEEN METHICILLIN-RESISTANT AND METHICILLIN-SUSCEPTIBLE ISOLATES OF STAPHYLOCOCCUS AUREUS. IRANIAN BIOMEDICAL JOURNAL. 2016 [cited 2021July25];20(3):175-181. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511666



IEEE: Copy

GHASEMIAN, A., NAJAR PEERAYEH, S., BAKHSHI, B., MIRZAEE, M., 2016. COMPARISON OF BIOFILM FORMATION BETWEEN METHICILLIN-RESISTANT AND METHICILLIN-SUSCEPTIBLE ISOLATES OF STAPHYLOCOCCUS AUREUS. IRANIAN BIOMEDICAL JOURNAL, [online] 20(3), pp.175-181. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511666.



 
  pdf-File
Yearly Visit 48
 
 
Latest on Blog
Enter SID Blog