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Paper Information

Journal:   IRANIAN BIOMEDICAL JOURNAL   JULY 2016 , Volume 20 , Number 3; Page(s) 161 To 167.
 
Paper: 

CYTOPROTECTIVE EFFECT OF HYDROALCOHOLIC EXTRACT OF PINUS ELDARICA BARK AGAINST H2O2-INDUCED OXIDATIVE STRESS IN HUMAN ENDOTHELIAL CELLS

 
DOI: 

10.7508/ibj.2016.03.005

 
Author(s):  BABAEE FATEMEH, SAFAEIAN LEILA*, ZOLFAGHARI BEHZAD, HAGHJOO JAVANMARD SHAGHAYEGH
 
* DEPT. OF PHARMACOLOGY AND TOXICOLOGY, ISFAHAN PHARMACEUTICAL SCIENCES RESEARCH CENTER, SCHOOL OF PHARMACY AND PHARMACEUTICAL SCIENCES, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Background: Pinus eldarica is a widely growing pine in Iran consisting of biologically active constituents with antioxidant properties. This study investigates the effect of hydroalcoholic extract of P. eldarica bark against oxidative damage induced by hydrogen peroxide (H2O2) in human umbilical vein endothelial cells (HUVECs). Methods: The total phenolic content of P. eldarica extract was determined using Folin-Ciocalteu method. The cytotoxicity of P. eldarica extract (25-1000 mg/ml) on HUVECs was assessed using 3-(4, 5- Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method. Cytoprotective effect of P. eldarica extract (25-500 mg/ml) on H2O2-induced oxidative stress was also evaluated by MTT assay. The intra- and extra-cellular hydroperoxides concentration and ferric reducing antioxidant power (FRAP) were measured in pretreated cells.
Results: The total phenolic content of P. eldarica extract was estimated as 37.04±1.8% gallic acid equivalent. P. eldarica extract (25-1000
mg/ml) had no cytotoxic effect on HUVECs viability. The pretreatment of HUVECs with P. eldarica extract at the concentrations of 50-500 mg/ml significantly reduced the cytotoxicity of H2O2. P. eldarica extract decreased hydroperoxides concentration and increased FRAP value in intra-cellular fluid at the concentration range of 100-500 mg/ml and in extra-cellular fluid at the concentration range of 25-500 mg/ml. Conclusions: This study revealed the antioxidant and cytoprotective effects of P. eldarica extract against H2O2-induced oxidative stress in HUVECs. Concerning the high content of phenolic compounds in P. eldarica, more research is needed to evaluate its clinical value in endothelial dysfunction and in other oxidative conditions.

 
Keyword(s): PINUS ELDARICA, HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS, OXIDATIVE STRESS, ANTIOXIDANTS
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

BABAEE, F., & SAFAEIAN, L., & ZOLFAGHARI, B., & HAGHJOO JAVANMARD, S. (2016). CYTOPROTECTIVE EFFECT OF HYDROALCOHOLIC EXTRACT OF PINUS ELDARICA BARK AGAINST H2O2-INDUCED OXIDATIVE STRESS IN HUMAN ENDOTHELIAL CELLS. IRANIAN BIOMEDICAL JOURNAL, 20(3), 161-167. https://www.sid.ir/en/journal/ViewPaper.aspx?id=511659



Vancouver: Copy

BABAEE FATEMEH, SAFAEIAN LEILA, ZOLFAGHARI BEHZAD, HAGHJOO JAVANMARD SHAGHAYEGH. CYTOPROTECTIVE EFFECT OF HYDROALCOHOLIC EXTRACT OF PINUS ELDARICA BARK AGAINST H2O2-INDUCED OXIDATIVE STRESS IN HUMAN ENDOTHELIAL CELLS. IRANIAN BIOMEDICAL JOURNAL. 2016 [cited 2021July31];20(3):161-167. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511659



IEEE: Copy

BABAEE, F., SAFAEIAN, L., ZOLFAGHARI, B., HAGHJOO JAVANMARD, S., 2016. CYTOPROTECTIVE EFFECT OF HYDROALCOHOLIC EXTRACT OF PINUS ELDARICA BARK AGAINST H2O2-INDUCED OXIDATIVE STRESS IN HUMAN ENDOTHELIAL CELLS. IRANIAN BIOMEDICAL JOURNAL, [online] 20(3), pp.161-167. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511659.



 
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