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Paper Information

Journal:   IRANIAN JOURNAL OF PATHOLOGY (IJP)   SUMMER 2016 , Volume 11 , Number 3; Page(s) 238 To 247.
 
Paper: 

SPECIES-SPECIFIC PCR FOR THE DIAGNOSIS AND DETERMINATION OF ANTIBIOTIC SUSCEPTIBILITIES OF BRUCELLA STRAINS ISOLATED FROM TEHRAN, IRAN

 
 
Author(s):  IRAJIAN GHOLAM REZA, MASJEDIAN JAZI FARAMARZ*, MIRNEJAD REZA, PIRANFAR VAHHAB, ZAHRAEI SALEHI TAGHI, MOZAFARI NOOR AMIR, GHAZNAVI RAD EHSANOLLAH, KHORMALI MAHMOOD
 
* DEPT. OF MICROBIOLOGY, IRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: Brucellosis is an endemic zoonotic disease in the Middle East. This study intended to design a uniplex PCR assay for the detection and differentiation of Brucella at the species level and determining the antibiotic susceptibility pattern of Brucella in Iran.
Methods: Sixty-eight Brucella specimens (38 animal and 30 human specimens) were analyzed using PCR (using one pair of primers). Antibiotic susceptibility patterns were evaluated and compared using the E-Test and disk diffusion susceptibility test. Tigecycline susceptibility pattern was compared with other antibiotics.
Results: Thirty six isolates of B. melitensis, 2 isolates of B. abortus and 1 isolate of B. suis from the 38 animal specimens, 24 isolates of B. melitensis and 6 isolates of B. abortus from the 30 human specimens were differentiated. The MIC50 values of doxycycline for human and animal specimens were 125 and 10
mg/ml, respectively, Tigecycline 0.064 mg/ml for human specimens and 0.125mg/ml for animal specimens, and Trimethoprim/Sulfamethoxazole and Ciprofloxacin 0.065 and 0.125mg/ml, respectively, for both human and animal specimens. The highest MIC50 value of streptomycin in the human specimens was 0.5mg/ml and 1mg/ml for the animal specimens. The greatest resistance shown was to tetracycline and Gentamicin, respectively.
Conclusion: Uniplex PCR for the detection and differentiation of Brucella at the strain level is faster and less expensive than multiplex PCR, and the antibiotics Doxycycline, Rifampin, Trimethoprim/Sulfamethoxazole, Ciprofloxacin, and Ofloxacin are the most effective antibiotics for treating brucellosis. Resistance to Tigecycline is increasing, and we recommend that it be used in a combination regimen.

 
Keyword(s): UNIPLEX PCR, BRUCELLA, ANTIBIOTIC SUSCEPTIBILITIES, TIGECYCLINE
 
 
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APA: Copy

IRAJIAN, G., & MASJEDIAN JAZI, F., & MIRNEJAD, R., & PIRANFAR, V., & ZAHRAEI SALEHI, T., & MOZAFARI, N., & GHAZNAVI RAD, E., & KHORMALI, M. (2016). SPECIES-SPECIFIC PCR FOR THE DIAGNOSIS AND DETERMINATION OF ANTIBIOTIC SUSCEPTIBILITIES OF BRUCELLA STRAINS ISOLATED FROM TEHRAN, IRAN. IRANIAN JOURNAL OF PATHOLOGY (IJP), 11(3), 238-247. https://www.sid.ir/en/journal/ViewPaper.aspx?id=511618



Vancouver: Copy

IRAJIAN GHOLAM REZA, MASJEDIAN JAZI FARAMARZ, MIRNEJAD REZA, PIRANFAR VAHHAB, ZAHRAEI SALEHI TAGHI, MOZAFARI NOOR AMIR, GHAZNAVI RAD EHSANOLLAH, KHORMALI MAHMOOD. SPECIES-SPECIFIC PCR FOR THE DIAGNOSIS AND DETERMINATION OF ANTIBIOTIC SUSCEPTIBILITIES OF BRUCELLA STRAINS ISOLATED FROM TEHRAN, IRAN. IRANIAN JOURNAL OF PATHOLOGY (IJP). 2016 [cited 2021July29];11(3):238-247. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511618



IEEE: Copy

IRAJIAN, G., MASJEDIAN JAZI, F., MIRNEJAD, R., PIRANFAR, V., ZAHRAEI SALEHI, T., MOZAFARI, N., GHAZNAVI RAD, E., KHORMALI, M., 2016. SPECIES-SPECIFIC PCR FOR THE DIAGNOSIS AND DETERMINATION OF ANTIBIOTIC SUSCEPTIBILITIES OF BRUCELLA STRAINS ISOLATED FROM TEHRAN, IRAN. IRANIAN JOURNAL OF PATHOLOGY (IJP), [online] 11(3), pp.238-247. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511618.



 
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