Paper Information

Journal:   IRANIAN JOURNAL OF PATHOLOGY (IJP)   SUMMER 2016 , Volume 11 , Number 3; Page(s) 222 To 230.
 
Paper: 

DESIGNING AND DEVELOPMENT OF A DNA VACCINE BASED ON STRUCTURAL PROTEINS OF HEPATITIS C VIRUS

 
 
Author(s):  TEIMOURPOUR ROGHAYEH, TAJANI AMINEH SADAT, ASKARI VAHID REZA, ROSTAMI SINA, MESHKAT ZAHRA*
 
* ANTIMICROBIAL RESISTANCE RESEARCH CENTER, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, IRAN
 
Abstract: 

Background: Hepatitis C virus (HCV) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. Therefore, designing new and effective therapeutics is of great importance. The aim of the current study was to construct a DNA vaccine containing structural proteins of HCV and evaluation of its expression in an eukaryotic system.
Methods: Structural proteins of HCV (core, E1, and E2) were isolated and amplified from JFH strain of HCV genotype 2a using PCR method. The PCR product was cloned into pCDNA3.1 (+) vector and finally were confirmed by restriction enzyme analysis and sequencing methods. The eukaryotic expression of the vector was confirmed by RT-PCR.
Results: A recombinant vector containing 2241bp fragment of HCV structural genes was constructed. The desired plasmid was sequenced and corresponded to 100% identity with the submitted sequences in GenBank. RT-PCR results indicated that the recombinant plasmid could be expressed efficiently in the eukaryotic expression system.
Conclusion: Successful cloning of structural viral genes in pCDNA3.1 (+) vector and their expression in an eukaryotic expression system facilitates the development of new DNA vaccines against HCV. A DNA vaccine encoding core-E1-E2 antigens was designed. The desired expression vector can be used for further attempts in the development of vaccines.

 
Keyword(s): HCV, STRUCTURAL PROTEINS, DNA VACCINE
 
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