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Paper Information

Journal:   IRANIAN JOURNAL OF PATHOLOGY (IJP)   SPRING 2016 , Volume 11 , Number 2; Page(s) 112 To 119.
 
Paper: 

DESIGNING AND CONSTRUCTION OF A DNA VACCINE ENCODING TB10.4 GENE OF MYCOBACTERIUM TUBERCULOSIS

 
 
Author(s):  RASHIDIAN SAMIRA, TEIMOURPOUR ROGHAYEH, MESHKAT ZAHRA*
 
* ANTIMICROBIAL RESISTANCE RESEARCH CENTER, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, IRAN
 
Abstract: 

Background: Tuberculosis (TB) remains as a major cause of death. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning oftb10.4 gene in pcDNA3.1+plasmid and evaluation of its expression in eukaryotic cells.
Methods: Firstly, tb10.4 fragment was amplified by PCR and the PCR product was digested with restriction enzymes. Next, it was cloned into pcDNA3.1+plasmid.
Following that, pcDNA3.1+/tb10.4 recombinant plasmid was transfected into eukaryotic cells.
Results: 5700 bp band for pcDNA3.1+/ tb10.4 recombinant plasmid and 297 bp fragment fortb10.4 were observed. Cloning and transfection were successful and designed recombinant vector was confirmed by sequencing.
Conclusion: Successful cloning provides a basis for the development of new DNA vaccines against tuberculosis.

 
Keyword(s): MYCOBACTERIUM TUBERCULOSIS, TB10.4, DNA VACCINE
 
 
References: 
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Citations: 
 
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APA: Copy

RASHIDIAN, S., & TEIMOURPOUR, R., & MESHKAT, Z. (2016). DESIGNING AND CONSTRUCTION OF A DNA VACCINE ENCODING TB10.4 GENE OF MYCOBACTERIUM TUBERCULOSIS. IRANIAN JOURNAL OF PATHOLOGY (IJP), 11(2), 112-119. https://www.sid.ir/en/journal/ViewPaper.aspx?id=511336



Vancouver: Copy

RASHIDIAN SAMIRA, TEIMOURPOUR ROGHAYEH, MESHKAT ZAHRA. DESIGNING AND CONSTRUCTION OF A DNA VACCINE ENCODING TB10.4 GENE OF MYCOBACTERIUM TUBERCULOSIS. IRANIAN JOURNAL OF PATHOLOGY (IJP). 2016 [cited 2021July30];11(2):112-119. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511336



IEEE: Copy

RASHIDIAN, S., TEIMOURPOUR, R., MESHKAT, Z., 2016. DESIGNING AND CONSTRUCTION OF A DNA VACCINE ENCODING TB10.4 GENE OF MYCOBACTERIUM TUBERCULOSIS. IRANIAN JOURNAL OF PATHOLOGY (IJP), [online] 11(2), pp.112-119. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511336.



 
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