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Paper Information

Journal:   ARCHIVES OF IRANIAN MEDICINE   JUNE 2016 , Volume 19 , Number 6; Page(s) 420 To 425.
 
Paper: 

DECREASED EXPRESSION OF BIOINFORMATICALLY PREDICTED PIWIL2-TARGETTING MICRORNAS, MIR-1267 AND MIR-2276 IN BREAST CANCER

 
DOI: 

0161906/AIM.009

 
Author(s):  TORKASHVAND S., DAMAVANDI Z., MIRZAEI B., TAVALLAEI M., VASEI M., MOWLA SEYED JAVAD*
 
* MOLECULAR GENETICS DEPARTMENT, FACULTY OF BIOLOGICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

PURPOSE: Human Piwil2, a member of Piwi subfamily of Argonaute proteins, is primarily expressed in testis, where it regulates self-renewal of germ cells. However, its ectopic expression has been reported with several tumors, including breast cancer. The upregulation of piwil2 in various stages of breast cancer suggested its suitability as a novel tumor biomarker. Considering the vital role of microRNAs (miRNAs) in regulating the expression of most human genes, we hypothesized a concomitant downregulation of the bioinformatically-predicted piwil2-targetting microRNAs in breast cancer.
METHOD: We employed different bioinformatic tools to predict piwil2-targeting miRNAs. Then, from the list of predicted miRNAs, we chose two less studied miRNAs (miR-1267 and miR-2276) for experimental validation. Using a real-time RT-PCR approach, we quantified the relative expression of the miRNAs in 31 pairs of formalin-fixed paraffin-embedded tumor/non-tumor tissue samples.
RESULTS: Our data revealed a noticeable but not statistically significant (P=0.133) down regulation of miR-1267 in tumor samples, compared to non-tumor samples obtained from the same patients. Down regulation of miR-1267 was more significant in higher grades of malignancies (fold change = 2.39, P=0.033) and also in lymph nodes containing high-grade tumor cells (fold change = 6.66, P=0.02). Interestingly, a significant upregulation of miR-1267 was observed in tumors at high stages (stage 3a, 3b), compared to low stages (stage 2a, 2b) (fold change = 8.05, P=0.048). Similar patterns of expression alteration were also observed for miR-2276.
CONCLUSION: Altogether, our findings suggest a probable tumor suppressor role for miR-1267 and miR-2276 in breast tumor initiation and progression, but a probable promoting role for them in invasion and metastasis.

 
Keyword(s): BIOMARKER, BREAST CANCER, GENE EXPRESSION, MICRORNAS, PIWIL2
 
 
References: 
 
Citations: 
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APA: Copy

TORKASHVAND, S., & DAMAVANDI, Z., & MIRZAEI, B., & TAVALLAEI, M., & VASEI, M., & MOWLA, S. (2016). DECREASED EXPRESSION OF BIOINFORMATICALLY PREDICTED PIWIL2-TARGETTING MICRORNAS, MIR-1267 AND MIR-2276 IN BREAST CANCER. ARCHIVES OF IRANIAN MEDICINE, 19(6), 420-425. https://www.sid.ir/en/journal/ViewPaper.aspx?id=511010



Vancouver: Copy

TORKASHVAND S., DAMAVANDI Z., MIRZAEI B., TAVALLAEI M., VASEI M., MOWLA SEYED JAVAD. DECREASED EXPRESSION OF BIOINFORMATICALLY PREDICTED PIWIL2-TARGETTING MICRORNAS, MIR-1267 AND MIR-2276 IN BREAST CANCER. ARCHIVES OF IRANIAN MEDICINE. 2016 [cited 2021August04];19(6):420-425. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511010



IEEE: Copy

TORKASHVAND, S., DAMAVANDI, Z., MIRZAEI, B., TAVALLAEI, M., VASEI, M., MOWLA, S., 2016. DECREASED EXPRESSION OF BIOINFORMATICALLY PREDICTED PIWIL2-TARGETTING MICRORNAS, MIR-1267 AND MIR-2276 IN BREAST CANCER. ARCHIVES OF IRANIAN MEDICINE, [online] 19(6), pp.420-425. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=511010.



 
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