Paper Information

Journal:   IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY   WINTER 2010 , Volume 10 , Number 4; Page(s) 305 To 316.
 
Paper: 

REGULATION OF α-DOX1 , α-DOX3 .LE 16 AND CLECLLD15 GENES EXPRESSION FOLLOWING OXIDATIVE STRESSES IN TOMATO SHOOT

 
 
Author(s):  DEHDARI A.*
 
* FACULTY OF AGRICULTURE, UNIVERSITY OF YASOUJ, YASOUJ, I.R. IRAN
 
Abstract: 

In order to determine the effects of number of oxidative stresses on regulation and expression of a-DOX1 .a-DOX3 Le 16 and clEc11DI5 genes in tomato shoot tissue this study was conducted under greenhouse (soil culture) and hydroponic culture conditions. Three tomato genotypes including wild type ‘Ailsa Craig’ CAC’), the near isogenic ABA deficient ‘Flacca’ (‘Fie’) strain and the ethylene receptor mutant ‘Never ripe’ (Nr’) were used. The tomato plants were subjected to different types of oxidative stresses. Shoot tissue of stress-treated plants were harvested and subjected to RNR extraction and northern hybridization analysis with probes of above mentioned genes. Results showed that Le 16 gene up regulated only by salt and drought stresses in ‘AC’ and ‘Nr’ genotypes but not in ‘Flc’ indicating ethylene-dependent of Le 16 gene. Over expression of this gene in ‘Nr’ cultivar was increased by increasing salinity levels and plant growth stage but ‘AC’ cultivar showed reverse response. a-DOX1 up regulated by H2O2 and wounding treatments. This is the first report of over expression of a-DOX1 in the shoot tissue of tomato plants. Up regulation of a-DOX3 was detected following jasmonic acid and paraquat treatments in leaves of ‘Fie’ but not in the others, indicating that abscisic acid is a key regulator of a-DOX3 expression. Although cLEC11 DI5 gene expressed in leaves of above three genotypes by all treatments but over expression was observed only in ‘Fie’ by jasmonic acid and paraquat. So, up regulation of cLEC11DI5 gene in leaves of treated plants was ABA- dependent. In general, this study showed that Le 16, cLEC11D15 and a-DO3 up regulated in leaves of tomato plants by a number of oxidative stresses. In contracts with previous reports a-DOX1 up regulated in leaves of ‘AC’ cultivar in response to H2O2 and wounding stresses. Probably by transferring of H2O2 from roots to shoot and producing of H2O2 by wounding.

 
Keyword(s): α-DOX, LE 16, GENE EXPRESSION, LEAF, OXIDATIVE STRESSES, TOMATO
 
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