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Paper Information

Journal:   RESEARCH JOURNAL OF PHARMACOGNOSY   2016 , Volume 3 , Number 2; Page(s) 53 To 59.
 
Paper: 

THE EFFECT OF TEMPERATURE AND PH ON BIOMASS AND BIOACTIVE COMPOUNDS PRODUCTION IN SILYBUM MARIANUM HAIRY ROOT CULTURES

 
 
Author(s):  RAHIMI SH., HASANLOO T.*
 
* DEPARTMENT OF MOLECULAR PHYSIOLOGY, AGRICULTURAL BIOTECHNOLOGY RESEARCH INSTITUTE OF IRAN, AGRICULTURAL RESEARCH, EDUCATION AND EXTENSION ORGANIZATION (AREEO), KARAJ, IRAN
 
Abstract: 

Background and objectives: The seed extract of Silybum marianum contains seven flavonolignans known collectively as silymarin. These metabolites can be produced in hairy root cultures of S. marianum. The effect of different physical factors can change root biomass and silymarin production which has been investigated in the present study.
Methods: The effect of different physical factors of temperature (30oC/25oC, 25oC/25oC and 15oC/20oC in 16 h/8 h cycle) and pH (5, 5.7, 6 and 7) were evaluated with respect to the root biomass and silymarin production in hairy root cultures of the plant.
Results: Incubation temperature, 25 oC /25oC promoted the silymarin production in 4-week old hairy roots (0.18 mg/g DW) as compared with the cultures treated with 15oC/20oC and 30oC/25oC (0.13 and 0.12 mg/g DW, respectively). Maximal increases in biomass and silymarin accumulation occurred in the root cultures grown in pH 5 and 25oC/25oC (0.45 g and 0.26 mg/g DW). The content of silybin, isosilybin, silychristin, silydianin were 0.025, 0.024, 0.061 and 0.095 mg/g DW, respectively which were higher than those grown in higher pH.
Conclusion: The results of the present study suggest that 25oC/25oC and acidic environment of medium are beneficial for silymarin production using hairy root cultures. Furthermore, lipoxygenase (LOX) activity was strongly affected by pH which suggested that acidic environment may act as inducing signal for LOX activity and subsequently greater silymarin production.

 
Keyword(s): HAIRY ROOT, PH, SILYBUM MARIANUM, SILYMARIN, TEMPERATURE
 
 
References: 
 
Citations: 
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APA: Copy

RAHIMI, S., & HASANLOO, T. (2016). THE EFFECT OF TEMPERATURE AND PH ON BIOMASS AND BIOACTIVE COMPOUNDS PRODUCTION IN SILYBUM MARIANUM HAIRY ROOT CULTURES. RESEARCH JOURNAL OF PHARMACOGNOSY, 3(2), 53-59. https://www.sid.ir/en/journal/ViewPaper.aspx?id=510969



Vancouver: Copy

RAHIMI SH., HASANLOO T.. THE EFFECT OF TEMPERATURE AND PH ON BIOMASS AND BIOACTIVE COMPOUNDS PRODUCTION IN SILYBUM MARIANUM HAIRY ROOT CULTURES. RESEARCH JOURNAL OF PHARMACOGNOSY. 2016 [cited 2021September26];3(2):53-59. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510969



IEEE: Copy

RAHIMI, S., HASANLOO, T., 2016. THE EFFECT OF TEMPERATURE AND PH ON BIOMASS AND BIOACTIVE COMPOUNDS PRODUCTION IN SILYBUM MARIANUM HAIRY ROOT CULTURES. RESEARCH JOURNAL OF PHARMACOGNOSY, [online] 3(2), pp.53-59. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510969.



 
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