Paper Information

Journal:   ACTA MEDICA IRANICA   2016 , Volume 54 , Number 2; Page(s) 85 To 95.
 
Paper: 

STUDY OF SILYMARIN AND VITAMIN E PROTECTIVE EFFECTS ON SILVER NANOPARTICLE TOXICITY ON MICE LIVER PRIMARY CELL CULTURE

 
 
Author(s):  FAEDMALEKI FIROUZ, SHIRAZI FARSHAD H., EJTEMAEIMEHR SHAHRAM, ANJARANI SOGHRA, SALARIAN AMIR AHMAD, AHMADI ASHTIANI HAMIDREZA, RASTEGAR HOSSEIN*
 
* COSMETIC PRODUCTS RESEARCH CENTER, IRAN FOOD AND DRUG ADMINSTRATION, MINISTRY OF HEALTH AND MEDICAL EDUCATION, TEHRAN, IRAN
 
Abstract: 

Nanotechnology is a most promising field for generating new applications in medicine, although, only few nano products are currently in use for medical purposes. A most prominent nanoproduct is nanosilver. Nanosilver has biological properties which are significant for consumer products, food technology, textiles, and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging). For their antibacterial activity, silver nanoparticles (Ag NPs) are largely used in various commercially available products. The use of nano-silver is becoming more and more widespread in medicine and related applications, and due to its increasing exposure, toxicological and environmental issues need to be raised. Cytotoxicity induced by silver nanoparticles (AgNPs) and the role that oxidative stress plays in this process were demonstrated in human hepatoma cells AgNPs agglomerated in the cytoplasm and nuclei of treated cells, and they induced intracellular oxidative stress. AgNP reduced ATP content of the cell and caused damage to mitochondria and increased production of reactive oxygen species (ROS) in a dose-dependent manner. Silymarin was known as a hepatoprotective agent that is used in the treatment of hepatic diseases including viral hepatitis, alcoholic liver diseases, Amanita mushroom poisoning, liver cirrhosis, toxic and drug-induced liver diseases. It promotes protein synthesis, helps in regenerating liver tissue, controls inflammation, enhances glucuronidation, and protects against glutathione depletion. Vitamin E is a well-known antioxidant and has hepatoprotective effect in liver diseases. In this study, we investigated the cytotoxic effects of Ag NPs on primary liver cells of mice. Cell viability (cytotoxicity) was examined with MTT assay after primary liver cells of mice exposure to Ag NPs at 1, 10, 50, 100, 150, 200, 400 ppm for 24h. AgNPs caused a concentration- dependent decrease of cell viability (IC50 value=121.7 ppm or mg/ml). Then the hepatoprotective effect of silymarin and vitamin E were experimented on silver nanoparticle toxicity on mice liver primary cell culture. The results showed that silymarin at 600mg/ml and vitamin E at 2500mmol/l have protective effects on silver nanoparticle toxicity on mice liver primary cell culture. Viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles at 121.7ppm and co-treatment of silymarin, and vitamin E is more than viability percentage of the primary liver cell of the mouse were exposed to silver nanoparticles and silymarin or silver nanoparticles and vitamin E.

 
Keyword(s): SILVER NANOPARTICLE, SILYMARIN, VITAMIN E, MTT ASSAY
 
References: 
 
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