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Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   1ST WEEK AUGUST 2016 , Volume 34 , Number 385; Page(s) 649 To 656.
 
Paper: 

EVALUATION OF RELATIVE FREQUENCY OF SINGLE NUCLEOTIDE POLYMORPHISM G870A (RS9344) IN CYCLIND1 GENE IN COLORECTAL CANCER PATIENTS AND HEALTHY SUBJECTS OF ISFAHAN PROVINCE, IRAN

 
 
Author(s):  RAHIMIRAD SHIMA, SALEHI RASOUL*, SAFFAR BEHNAZ, NIAEE MOSTAFA
 
* DEPARTMENT OF GENETICS AND MOLECULAR BIOLOGY, SCHOOL OF MEDICINE, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Background: Colorectal cancer (CRC) is one of the most common cancers in the world. High activity of CyclinD1 gene has been seen in the progress of this cancer. A common polymorphism (G870A) in exon 4 of CyclinD1 produces a variant transcript with longer half-life and may cause uncontrollable cellular growth thus contributing to cancer development. This study was performed to evaluate the frequency of CCND1 G870A polymorphism between CRC cases and controls.
Methods: DNA samples from peripheral blood leukocytes of 50 patients with sporadic colorectal cancer and 50 healthy subjects were extracted. CCND1 G870A polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in healthy subjects and patients. Statistical analysis was performed by chi-squared test via SPSS software.
Findings: Relationship between CCND1 G870A polymorphism in allele frequencies between cases and controls were not observed (P=0.204). While the frequency of AA genotype was significantly higher in patients (P=0.040, 95% CI=1.13-5.54, OR=2.25).
Conclusion: According to the significant association observed between certain genotypes of this locus (AA) with colorectal cancer, it can be used in the future as a good prognostic marker in CRC screening programs.

 
Keyword(s): COLORECTAL CANCER, SINGLE NUCLEOTIDE POLYMORPHISM, CYCLIN D1 GENE
 
References: 
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