Paper Information

Journal:   JOURNAL OF PLANT PROCESS AND FUNCTION   SPRING 2016 , Volume 5 , Number 15; Page(s) 107 To 122.
 
Paper: 

BIOCHEMICAL CHARACTERIZATION OF ARABIDOPSIS NTRC MUTANTS DURING DARK- INDUCED SENESCENCE

 
 
Author(s):  TAMASKANI FATEMEH, SADEGHIPOUR HAMID REZA*, AGHDASI MAHNAZ, ABDOLZADEH AHMAD
 
* DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCE, GOLESTAN UNIVERSITY, GORGAN, IRAN
 
Abstract: 

Thioredoxins are involved in redox regulation of many cellular "processes. In this study the role of NADP+- Thioredoxin reductase C (NTRC) in the: control of leaf senescence was investigated by biochemical characterization of Arabidopsis ntrc mutants, Forty days. oldwild type and two ntrc mutant lines were incubated either under normal dark-light or contino us darkness regimes for 6 days as control and senescence treatment, respectively. Growth parameters, the contents of carbohydrates, pigments and the activity of some antioxidant enzymes were compared between wild type and ntrc mutant lines during dark induced senescence. Darkness led to decreased fresh and dry weights, chlorophylls and carotenoides, starch and protein contents in both wild type and mutant plants and there were no significant differences in the interactions of genotypes and treatments regarding to theses parameters. Darkness also induced the accumulation of anthocyan ins, total and non-reducing sugars however, the accumulation of the carbohydrates in mutant lines was more pronounciated than wild type plants and again there were no significant differences in response of wild type and mutants to dark treatment. The activities of catalase, peroxidase and polyphenol oxidase of mutant lines were significantly greater than wild ype plants. While darkness decreased the activities of ascorbate peroxidase and catalase it led to increased activities of peroxidase and polyphenol oxidase especially in the mutant lines. There were significant differences in the activities of all four enzymes between mutant and wild type plants in response to darkness. Thus, it appears that defects in NTRC mainly affects the activities of antioxidant enzymes rather than the other studied biochemical parameters of plants during the senescence process. This study might be useful in future breeding programs for controlling senescence process to achieve better performance of crop plants.

 
Keyword(s): ANTIOXIDANT ENZYMES, ANTHOCYANIN, CHLOROPHYLL, NTRC MUTANT, SENESCENCE, THIOREDOXIN
 
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