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Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   4TH WEEK JULY 2016 , Volume 34 , Number 384; Page(s) 589 To 593.
 
Paper: 

SENSITIVITY AND SPECIFICITY OF DOT BLOT METHOD FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION

 
 
Author(s):  SALEHI FARZAD, JAFARI RASOOL, SHARAFI SEYEDEH MARYAM, YOUSEFI HOSEINALI, PESTEHCHIAN NADER, YOUSEFI DARANI HOSEIN*
 
* INFECTIOUS DISEASES AND TROPICAL MEDICINE RESEARCH CENTER, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Background: Diagnosis of cryptosporidiosis is based on the use of the routine microscopic methods, but these methods possess poor sensitivity and also need an expert technician. The aim of the present study was to determine sensitivity and specificity of dot blot method for diagnosis of bovine cryptosporidiosis.
Methods: In this study 131 fecal samples were collected from suckling calves during summer 2014. The collected samples were concentrated using formalin ether method. The samples were then examined by modified-Ziehl-Neelsen (mZN) and finally all samples were examined by dot blot methods.
Findings: Oocysts of Cryptosporidium spp. were found in 40 (30.5%) out of 131 fecal samples using mZN staining method but 49 (37.4%) of the samples were positive for Cryptosporidium antigen using dot blot method.
No significant differences were observed among the results of the two methods and the sensitivity and specificity of dot blot calculated as 87% and 86%, respectively.
Conclusion: Based on the results of the present study, dot blot method showed rather equal performance as modified-Ziehl-Neelsen method.

 
Keyword(s): CRYPTOSPORIDIUM, MODIFIED-ZIEHL-NEELSEN, DOT BLOT, ANTIGEN DETECTION, SENSITIVITY, SPECIFICITY
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

SALEHI, F., & JAFARI, R., & SHARAFI, S., & YOUSEFI, H., & PESTEHCHIAN, N., & YOUSEFI DARANI, H. (2016). SENSITIVITY AND SPECIFICITY OF DOT BLOT METHOD FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 34(384), 589-593. https://www.sid.ir/en/journal/ViewPaper.aspx?id=510793



Vancouver: Copy

SALEHI FARZAD, JAFARI RASOOL, SHARAFI SEYEDEH MARYAM, YOUSEFI HOSEINALI, PESTEHCHIAN NADER, YOUSEFI DARANI HOSEIN. SENSITIVITY AND SPECIFICITY OF DOT BLOT METHOD FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S). 2016 [cited 2021July29];34(384):589-593. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510793



IEEE: Copy

SALEHI, F., JAFARI, R., SHARAFI, S., YOUSEFI, H., PESTEHCHIAN, N., YOUSEFI DARANI, H., 2016. SENSITIVITY AND SPECIFICITY OF DOT BLOT METHOD FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), [online] 34(384), pp.589-593. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510793.



 
 
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