Abstract:
Objective: MicroRNAs (miRNA) are a class of non-coding RNAs which play key roles in post-transcriptional gene regulation. Previous studies indicate that miRNAs are dysregulated in patients with multiple sclerosis (MS). Th17 and regulatory T (Treg) cells are two subsets of CD4+T-cells which have critical functions in the onset and progression of MS. The current study seeks to distinguish fluctuations in expression of CD4+T-cell derived miR-223 during the relapsing-remitting (RR) phase of MS (RR-MS), as well as the expressions of Th17 and Treg cell markers. Materials and Methods: This experimental study used real-time quantitative polymerase chain reaction (qRT-PCR) to evaluate CD4+T cell derived miR-223 expression patterns in patients that experienced either of the RR-MS phases (n=40) compared to healthy controls (n=12), along with RNA markers for Th17 and Treg cells. We conducted flow cytometry analyses of forkhead box P3 (FOXP3) and RAR-related orphan receptor ?t (ROR?t) in CD4+T-cells. Putative and validated targets of miR-223 were investigated in the miRWalk and miRTarBase databases, respectively. Results: miR-223 significantly upregulated in CD4+T-cells during the relapsing phase of RR-MS compared to the remitting phase (P=0.000) and healthy individuals (P=0.036). Expression ofROR?t, a master transcription factor of Th17, upregulated in the relapsing phase, whereasFOXP3 upregulated in the remitting phase. Additionally, potential targets of miR-223, STAT1, FORKHEAD BOX O (FOXO1) and FOXO3 were predicted by in silico studies. Conclusion: miR-223 may have a potential role in MS progression. Therefore, suppression of miR-223 can be proposed as an appropriate approach to control progression of the relapsing phase of MS.
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Cite:
APA: Copy
HOSSEINI, A., & GHAEDI, K., & TANHAEI, S., & GANJALIKHANI HAKEMI, M., & TEIMURI, S., & ETEMADIFAR, M., & NASRESFAHANI, M. (2016). UPREGULATION OF CD4+T-CELL DERIVED MIR-223 IN THE RELAPSING PHASE OF MULTIPLE SCLEROSIS PATIENTS. CELL JOURNAL (YAKHTEH), 18(3 (71)), 371-380. https://www.sid.ir/en/journal/ViewPaper.aspx?id=510756
Vancouver: Copy
HOSSEINI AREF, GHAEDI KAMRAN, TANHAEI SOMAYEH, GANJALIKHANI HAKEMI MAZDAK, TEIMURI SHOHREH, ETEMADIFAR MASOUD, NASRESFAHANI MOHAMMAD HOSSEIN. UPREGULATION OF CD4+T-CELL DERIVED MIR-223 IN THE RELAPSING PHASE OF MULTIPLE SCLEROSIS PATIENTS. CELL JOURNAL (YAKHTEH). 2016 [cited 2021April14];18(3 (71)):371-380. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510756
IEEE: Copy
HOSSEINI, A., GHAEDI, K., TANHAEI, S., GANJALIKHANI HAKEMI, M., TEIMURI, S., ETEMADIFAR, M., NASRESFAHANI, M., 2016. UPREGULATION OF CD4+T-CELL DERIVED MIR-223 IN THE RELAPSING PHASE OF MULTIPLE SCLEROSIS PATIENTS. CELL JOURNAL (YAKHTEH), [online] 18(3 (71)), pp.371-380. Available at: <https://www.sid.ir/en/journal/ViewPaper.aspx?id=510756>.
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