Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   FALL 2016 , Volume 18 , Number 3 (71); Page(s) 322 To 331.
 
Paper: 

EFFECT OF THE RECEPTOR ACTIVATOR OF NUCLEAR FACTOR KB AND RANK LIGAND ONIN VITRO DIFFERENTIATION OF CORD BLOOD CD133+ HEMATOPOIETIC STEM CELLS TO OSTEOCLASTS

 
 
Author(s):  KALANTARI NASIM, ABROUN SAEID*, SOLEIMANI MASOUD, KAVIANI SAEID, AZAD MEHDI, ESKANDARI FATEMEH, HABIBI HOSSEIN
 
* DEPARTMENT OF HEMATOLOGY AND BLOOD BANKING, FACULTY OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Objective: Receptor activator of nuclear factor-kappa B ligand (RANKL) appears to be an osteoclast-activating factor, bearing an important role in the pathogenesis of multiple myeloma. Some studies demonstrated that U-266 myeloma cell line and primary myeloma cells expressed RANK and RANKL. It had been reported that the expression of myeloid and monocytoid markers was increased by co-culturing myeloma cells with hematopoietic stem cells (HSCs). This study also attempted to show the molecular mechanism of RANK and RANKL on differentiation capability of human cord blood HSC to osteoclast, as well as expression of calcitonin receptor (CTR) on cord blood HSC surface.
Materials and Methods: In this experimental study, CD133+hematopoietic stem cells were isolated from umbilical cord blood and cultured in the presence of macrophage colony-stimulating factor (M-CSF) and RANKL. Osteoclast differentiation was characterized by using tartrate-resistant acid phosphatase (TRAP) staining, giemsa staining, immunophenotyping, and reverse transcription-polymerase chain reaction (RT-PCR) assay for specific genes.
Results: Hematopoietic stem cells expressed RANK before and after differentiation into osteoclast. Compared to control group, flow cytometric results showed an increased expression of RANK after differentiation. Expression ofCTR mRNA showed TRAP reaction was positive in some differentiated cells, including osteoclast cells.
Conclusion: Presence of RANKL and M-CSF in bone marrow could induce HSCs differentiation into osteoclast.

 
Keyword(s): RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B, RANK LIGAND, HEMATOPOIETIC STEM CELLS, OSTEOCLASTS, CALCITONIN RECEPTOR
 
References: 
 
  pdf-File tarjomyar Yearly Visit 73
 
Latest on Blog
Enter SID Blog