Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   FALL 2016 , Volume 18 , Number 3 (71); Page(s) 310 To 321.
 
Paper: 

IN VIVO DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS ON ELECTROSPUN POLY-L-LACTIDE ACID SCAFFOLDS COATED WITH MATRICARIA CHAMOMILLAL. OIL

 
 
Author(s):  FAZILI AFSANEH, GHOLAMI SOGHRA*, MINAIE ZANGI BAGHER, SEYEDJAFARI EHSAN, GHOLAMI MAHDI
 
* DEPARTMENT OF ANATOMY, SCHOOL OF VETERINARY MEDICINE, UNIVERSITY OF SHIRAZ, SHIRAZ, IRAN
 
Abstract: 

Objective: This study examined the in vivo differentiation of mesenchymal stem cells (MSCs) into insulin producing cells (IPCs) on electrospun poly-L-lactide acid (PLLA) scaffolds coated withMatricaria chammomila L. (chamomile) oil.
Materials and Methods: In this interventional, experimental study adipose MSCs (AMSCs) were isolated from 12 adult male New Zealand white rabbits and characterized by flow cytometry. AMSCs were subsequently differentiated into osteogenic and adipogenic lines. Cells were seeded onto either a PLLA scaffold (control) or PLLA scaffold coated with chamomile oil (experimental). A total of 24 scaffolds were inserted into the pancreatic area of each rabbit and placement was confirmed by ultrasound. After 21 days, immunohistochemistry analysis of insulin-producing like cells on protein levels confirmed insulin expression of insulin producing cells (IPSCs).
Real-time polymerase chain reaction (PCR) determined the expressions of genes related to pancreatic endocrine development and function.
Results: Fourier transform infrared spectroscopy (FTIR) results confirmed the existence of oil on the surface of the PLLA scaffold. The results showed a new peak at 2854 cm-1 for the aliphatic CH2 bond. Pdx1 expression was 0.051 ± 0.007 in the experimental group and 0.009 ± 0.002 in the control group. There was significantly increased insulin expression in the scaffold coated with chamomile oil (0.09 ± 0.001) compared to control group (0.063 ± 0.009, P?.05). Both groups expressedNgn3 and Pdx1specific markers and pancreatic tissue was observed at 21 days post transplantation.
Conclusion: The pancreatic region is an optimal site for differentiation of AMSCs to IPCs.
Chamomile oil (as an antioxidant agent) can affect cell adhesion to the scaffold and increase cell differentiation. In addition, the oil may lead to increased blood glucose uptake in pathways in the muscles, liver and fatty tissue of a diabetic animal model by some probable molecular mechanisms.

 
Keyword(s): DIFFERENTIATION, TRANSPLANTATION, SCAFFOLD, INSULIN
 
References: 
 
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