Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF MICROBIOLOGY   FEBRUARY 2016 , Volume 8 , Number 1; Page(s) 1 To 7.
 
Paper: 

A BIOASSAY-GUIDED FRACTIONATION SCHEME FOR CHARACTERIZATION OF NEW ANTIBACTERIAL COMPOUNDS FROM PROSOPIS CINERARIA AERIAL PARTS

 
 
Author(s):  NEGHABI HAJIAGHA MAHDIEH, ALIAHMADI ATOUSA, TAHERI MOHAMMADREZA, GHASSEMPOUR ALIREZA, IRAJIAN GHOLAMREZA, REZADOOST HASSAN, FEIZABADI MOHAMMADMEHDI*
 
* DEPARTMENT OF MICROBIOLOGY, SCHOOL OF MEDICINE, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, POORSINA STREET, TEHRAN, IRAN
 
Abstract: 

Background and Objectives: Due to the importance of finding of new antibacterial agents, the antibacterial properties of Prosopis cineraria aerial parts were investigated using a bioassay guided fractionation scheme.
Materials and Methods: The organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane and ethyl acetate. The MICs of fractions were determined against some human pathogenic bacteria using broth micro-dilution assay. The primary characterization and identification of bioactive substance(s) was based on a bio-autographical method using HPTLC and flash chromatography in parallel with agar overlay assays. Finally the exact mass of effective compound(s) was determined by LC-MS.
Results: The best antibacterial activities were related to the ethyl acetate fraction. The effective antibacterial compound of the plant were 2 substances with molecular weight of 348 and 184 Dalton that inhibited the growth of assessed Gram positive bacteria with MIC values lower than 125 to 62.5 ?g/ml synergistically.
Conclusion: Further analysis using nuclear magnetic resonance could reveal the exact structure of these two antibacterial substances. These 2 effective antibacterial compounds could be applied as lead compound for synthesis of new antibacterial agents.

 
Keyword(s): STAPHYLOCOCCUS AUREUS, PROSOPIS CINERARIA, ANTIBIOTIC RESISTANT, CHARACTERIZATION, BIOASSAY
 
 
References: 
  • Not Registered.
  •  
  •  
 
Citations: 
  • Not Registered.
 
+ Click to Cite.
APA: Copy

NEGHABI HAJIAGHA, M., & ALIAHMADI, A., & TAHERI, M., & GHASSEMPOUR, A., & IRAJIAN, G., & REZADOOST, H., & FEIZABADI, M. (2016). A BIOASSAY-GUIDED FRACTIONATION SCHEME FOR CHARACTERIZATION OF NEW ANTIBACTERIAL COMPOUNDS FROM PROSOPIS CINERARIA AERIAL PARTS. IRANIAN JOURNAL OF MICROBIOLOGY, 8(1), 1-7. https://www.sid.ir/en/journal/ViewPaper.aspx?id=510714



Vancouver: Copy

NEGHABI HAJIAGHA MAHDIEH, ALIAHMADI ATOUSA, TAHERI MOHAMMADREZA, GHASSEMPOUR ALIREZA, IRAJIAN GHOLAMREZA, REZADOOST HASSAN, FEIZABADI MOHAMMADMEHDI. A BIOASSAY-GUIDED FRACTIONATION SCHEME FOR CHARACTERIZATION OF NEW ANTIBACTERIAL COMPOUNDS FROM PROSOPIS CINERARIA AERIAL PARTS. IRANIAN JOURNAL OF MICROBIOLOGY. 2016 [cited 2021July24];8(1):1-7. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510714



IEEE: Copy

NEGHABI HAJIAGHA, M., ALIAHMADI, A., TAHERI, M., GHASSEMPOUR, A., IRAJIAN, G., REZADOOST, H., FEIZABADI, M., 2016. A BIOASSAY-GUIDED FRACTIONATION SCHEME FOR CHARACTERIZATION OF NEW ANTIBACTERIAL COMPOUNDS FROM PROSOPIS CINERARIA AERIAL PARTS. IRANIAN JOURNAL OF MICROBIOLOGY, [online] 8(1), pp.1-7. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510714.



 
  pdf-File
Yearly Visit 39
 
 
Latest on Blog
Enter SID Blog