Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)   JULY-SEPTEMBER 2016 , Volume 8 , Number 3; Page(s) 139 To 144.
 
Paper: 

THE EFFECT OF MEDIA SUPPLEMENTATION WITH ANGIOTENSIN ON DEVELOPMENTAL COMPETENCE OF OVINE EMBRYOS DERIVED FROM VITRIFIED-WARMED OOCYTES

 
 
Author(s):  NADERI MOHAMMAD MEHDI, BORJIAN BOROUJENI SARA, SARVARI ALI, HEYDARI BANAFSHEH, AKHONDI MOHAMMAD MEHDI, ZARNANI AMIR HASSAN, SHIRAZI ABOLFAZL*
 
* REPRODUCTIVE BIOTECHNOLOGY RESEARCH CENTER, AVICENNA RESEARCH INSTITUTE, ACECR, TEHRAN, IRAN
 
Abstract: 

Background: This study was aimed to assess the effects of angiotensin II (Ang II) supplementation to the In Vitro Maturation (IVM) and In Vitro Culture (IVC) media of vitrified-warmed ovine oocytes on their developmental competence and expression of Na+/K+/ATPase in resulting embryos.
Methods: The slaughterhouse-derived immature oocytes (n=1069) were randomly distributed into four experimental groups: groups I and II) IVM/IVF and IVC of fresh and vitrified oocytes without angiotensin supplementation (Control-Fresh and Control-Vit groups, respectively); group III) IVM of vitrified oocytes in the presence of Ang II followed by IVF/IVC (Vit-IVM group); and group IV) IVM/IVF of vitrified oocytes followed by IVC wherein the embryos were exposed to Ang II on day 4 of IVC (Vit-D4 group).
The embryos were immunostained with primary antibodies against Na+/K+/ATPase
a1 and b1 subunits.
Results: In Vit-IVM and Vit-D4 groups, the rates of expanded and total blastocysts on day 7 as well as the proportion of blastocysts on day 8 were increased. The expression of Na+/K+/ATPase
a1 and b 1 subunits were positively influenced by the addition of Ang II on day 4 (Vit-D4 group).
Conclusion: The addition of Ang II to the IVM and IVC media could improve blastocysts formation in vitrified sheep oocytes. This improvement might be related to the greater expression of Na+/K+/ATPase
a1 and b 1 subunits when Ang II was added during IVC.

 
Keyword(s): ANGIOTENSIN II, NA+/K+/ATPASE, OOCYTES, OVINE, VITRIFICATION
 
 
References: 
 
Citations: 
  • Not Registered.
 
+ Click to Cite.
APA: Copy

NADERI, M., & BORJIAN BOROUJENI, S., & SARVARI, A., & HEYDARI, B., & AKHONDI, M., & ZARNANI, A., & SHIRAZI, A. (2016). THE EFFECT OF MEDIA SUPPLEMENTATION WITH ANGIOTENSIN ON DEVELOPMENTAL COMPETENCE OF OVINE EMBRYOS DERIVED FROM VITRIFIED-WARMED OOCYTES. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 8(3), 139-144. https://www.sid.ir/en/journal/ViewPaper.aspx?id=510612



Vancouver: Copy

NADERI MOHAMMAD MEHDI, BORJIAN BOROUJENI SARA, SARVARI ALI, HEYDARI BANAFSHEH, AKHONDI MOHAMMAD MEHDI, ZARNANI AMIR HASSAN, SHIRAZI ABOLFAZL. THE EFFECT OF MEDIA SUPPLEMENTATION WITH ANGIOTENSIN ON DEVELOPMENTAL COMPETENCE OF OVINE EMBRYOS DERIVED FROM VITRIFIED-WARMED OOCYTES. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB). 2016 [cited 2021August04];8(3):139-144. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510612



IEEE: Copy

NADERI, M., BORJIAN BOROUJENI, S., SARVARI, A., HEYDARI, B., AKHONDI, M., ZARNANI, A., SHIRAZI, A., 2016. THE EFFECT OF MEDIA SUPPLEMENTATION WITH ANGIOTENSIN ON DEVELOPMENTAL COMPETENCE OF OVINE EMBRYOS DERIVED FROM VITRIFIED-WARMED OOCYTES. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), [online] 8(3), pp.139-144. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510612.



 
  pdf-File
Yearly Visit 44
 
 
Latest on Blog
Enter SID Blog