Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)   JULY-SEPTEMBER 2016 , Volume 8 , Number 3; Page(s) 126 To 132.
 
Paper: 

INHIBITORY EFFECTS OF SOME CARBOHYDRATES ON NANO-GLOBULAR AGGREGATION OF BOTH NORMAL AND GLYCATED ALBUMIN

 
 
Author(s):  MOUSAVI MOVAHEDI ALI AKBAR, SATTARAHMADY NAGHMEH*, SHARIFI ESMAEIL, HELI HOSSEIN
 
* DEPARTMENT OF MEDICAL PHYSICS, FACULTY OF MEDICINE, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SHIRAZ, IRAN
 
Abstract: 

Background: Protein aggregation is one of the important, common and troubling problems in biotechnology, pharmaceutical industries and amyloid-re-lated disorders.
Methods: In the present study, the inhibitory effects of some carbohydrates (alginate,
b-cyclodextrin and trehalose) on the formation of nano-globular aggregates from normal (HSA) and glycated (GHSA) human serum albumin were studied; when the formation of aggregates was induced by the simultaneous heating and addition of dithiotheritol. For the investigations, the biophysical methods of UV-vis spectrophotometry, circular dichroism spectroscopy, transmission electron microscopy and tensiometry were employed.
Results: The effect of inhibitory mechanism of these inhibitors on the aggregation of HSA and GHSA was expressed and compared together.
Conclusion: The results showed that the nucleus formation step of the aggregation process of HSA and GHSA was different in the presence of alginate (compared to?-cyclodextrin and trehalose). The inhibition efficiencies of the carbohydrates on the aggregate formation of HSA and GHSA were different, arising from the differences in the hydrophobicities of HSA and GHSA, and also, the differences between HSA- and GHSA-carbohydrate interactions.

 
Keyword(s): ALBUMIN, ALGINATE, PROTEIN AGGREGATION, TREHALOSE
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 104
 
Latest on Blog
Enter SID Blog