Paper Information

Journal:   AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)   JULY-SEPTEMBER 2016 , Volume 8 , Number 3; Page(s) 112 To 119.
 
Paper: 

THE CYTOTOXICITY OF DACARBAZINE POTENTIATED BY SEA CUCUMBER SAPONIN IN RESISTANT B16F10 MELANOMA CELLS THROUGH APOPTOSIS INDUCTION

 
 
Author(s):  BAHARARA JAVAD*, AMINI ELAHEH, NIKDEL NAJME, SALEK ABDOLLAHI FARZANEH
 
* RESEARCH CENTER FOR APPLIED BIOLOGY, MASHHAD BRANCH, ISLAMIC AZAD UNIVERSITY, MASHHAD, IRAN
 
Abstract: 

Background: Malignant melanoma is a highly aggressive malignant melanocytic neoplasm which resists against the most conventional therapies. Sea cucumber as one of marine organisms contains bioactive compounds such as polysaccharide, terpenoid and other metabolites which have anti-cancer, anti-tumor, anti-inflammatory and antioxidant properties. The present study was designed to investigate the anticancer potential of saponin extracted from sea cucumberHolothuria leucospilata alone and in combination with dacarbazine on B16F10 melanoma cell line.
Methods: The B16F10 cell line was treated with different concentrations of saponin (0, 4, 8, 12, 16, 20
mg/ml), dacarbazine (0, 1200, 1400, 1600, 1800, 2000 mg/ml) and coadministration of saponin-dacarbazine (1200 da+8 sp, 1200 da+4 sp) for 24 and 48hr and the cytotoxic effect was examined by MTT, DAPI, acridine orange/propodium iodide, flow cytometry and caspase colorimetric assay.
Results: The results exhibited that sea cucumber saponin, dacarbazine, and coadministration of saponin-dacarbazine inhibited the proliferation of melanoma cells in a dose and time dependent manner with IC50 values of 10, 1400 and 4+1200
mg/ml, respectively. Morphological observation of DAPI and acridine orange/propodium iodide staining documented typical characteristics of apoptotic cell death. Flow cytometry assay indicated accumulation of IC50 treated cells in sub-G1 peak. Additionally, saponin extracted induced intrinsic apoptosisvia up-regulation of caspase-3 and caspase-9.
Conclusion: These results revealed that the saponin extracted from sea cucumber as a natural anti-cancer compound may be a new treatment modality for metastatic melanoma and the application of sea cucumber saponin in combination with dacarbazine demonstrated the strongest anti-cancer activity as compared with the drug alone.

 
Keyword(s): APOPTOSIS, DACARBAZINE, MELANOMA, SAPONINS, SEA CUCUMBERS
 
References: 
 
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