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Paper Information

Journal:   IRANIAN JOURNAL OF MICROBIOLOGY   JUNE 2016 , Volume 8 , Number 3; Page(s) 193 To 202.
 
Paper: 

IMMUNOREACTIVITY EVALUATION OF A NEW RECOMBINANT CHIMERIC PROTEIN AGAINST BRUCELLA IN THE MURINE MODEL

 
 
Author(s):  ABDOLLAHI ABBAS, MANSOURI SHAHLA*, AMANI JAFAR*, FASIHI RAMANDI MAHDI, MORADI MOHAMMAD
 
* APPLIED MICROBIOLOGY RESEARCH CENTER, BAQIYATALLAH MEDICAL SCIENCES UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background and Objectives: Brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. Because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against Brucella is desirable. In this study, we evaluated the immune responses induced by a designed recombinant chimera protein in murine model.
Materials and Methods: Three immunodominant antigens of Brucella have been characterized as potential immunogenic and protective antigens including: trigger factor (TF), Omp31 and Bp26 were fused together by EAAAK linkers to produce a chimera (structure were designed in silico), which was synthesized, cloned, and expressed in E. coli BL21 (DE3). The purification of recombinant protein was performed using Ni-NTA agarose. SDS-PAGE and anti-His antibody was used for confirmation purified protein (Western blot). BALB/c immunization was performed by purified protein and adjuvant, and sera antibody levels were measured by ELISA. otted. Results: SDS-PAGE and Western blotting results indicated the similarity of in silico designing and in vitro experiments. ELISA result proved that the immunized sera of mice contain high levels of antibodies (IgG) against recombinant chimeric protein.
Conclusion: The recombinant chimeric protein could be a potential antigen candidate for the development of a subunit vaccine against Brucella.

 
Keyword(s): BRUCELLA, VACCINE, IMMUNITY, RECOMBINANT
 
 
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APA: Copy

ABDOLLAHI, A., & MANSOURI, S., & AMANI, J., & FASIHI RAMANDI, M., & MORADI, M. (2016). IMMUNOREACTIVITY EVALUATION OF A NEW RECOMBINANT CHIMERIC PROTEIN AGAINST BRUCELLA IN THE MURINE MODEL. IRANIAN JOURNAL OF MICROBIOLOGY, 8(3), 193-202. https://www.sid.ir/en/journal/ViewPaper.aspx?id=510593



Vancouver: Copy

ABDOLLAHI ABBAS, MANSOURI SHAHLA, AMANI JAFAR, FASIHI RAMANDI MAHDI, MORADI MOHAMMAD. IMMUNOREACTIVITY EVALUATION OF A NEW RECOMBINANT CHIMERIC PROTEIN AGAINST BRUCELLA IN THE MURINE MODEL. IRANIAN JOURNAL OF MICROBIOLOGY. 2016 [cited 2021July30];8(3):193-202. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510593



IEEE: Copy

ABDOLLAHI, A., MANSOURI, S., AMANI, J., FASIHI RAMANDI, M., MORADI, M., 2016. IMMUNOREACTIVITY EVALUATION OF A NEW RECOMBINANT CHIMERIC PROTEIN AGAINST BRUCELLA IN THE MURINE MODEL. IRANIAN JOURNAL OF MICROBIOLOGY, [online] 8(3), pp.193-202. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510593.



 
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