Paper Information

Journal:   IRANIAN JOURNAL OF ARTHROPOD-BORNE DISEASES   JUNE 2016 , Volume 10 , Number 2; Page(s) 127 To 140.
 
Paper: 

GENETIC DIVERSITY OF CRIMEAN CONGO HEMORRHAGIC FEVER VIRUS STRAINS FROM IRAN

 
 
Author(s):  CHINIKAR SADEGH*, BOUZARI SAEID, SHOKRGOZAR MOHAMMAD ALI, MOSTAFAVI EHSAN, JALALI TAHMINEH, KHAKIFIROUZ SAHAR, NOWOTNY NORBERT, FOOKS ANTHONY R., SHAH HOSSEINI NARIMAN
 
* ARBOVIRUSES AND VIRAL HEMORRHAGIC FEVERS LABORATORY (NATIONAL REFERENCE LABORATORY), PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 

Background: Crimean Congo hemorrhagic fever virus (CCHFV) is a member of the Bunyaviridae family and Nairovirus genus. It has a negative-sense, single stranded RNA genome approximately 19.2 kb, containing the Small, Medium, and Large segments. CCHFVs are relatively divergent in their genome sequence and grouped in seven distinct clades based on S-segment sequence analysis and six clades based on M-segment sequences. Our aim was to obtain new insights into the molecular epidemiology of CCHFV in Iran.
Methods: We analyzed partial and complete nucleotide sequences of the S and M segments derived from 50 Iranian patients. The extracted RNA was amplified using one-step RT-PCR and then sequenced. The sequences were analyzed using Mega5 software.
Results: Phylogenetic analysis of partial S segment sequences demonstrated that clade IV-(Asia 1), clade IV-(Asia 2) and clade V-(Europe) accounted for 80%, 4% and 14% of the circulating genomic variants of CCHFV in Iran respectively. However, one of the Iranian strains (Iran-Kerman/22) was associated with none of other sequences and formed a new clade (VII). The phylogenetic analysis of complete S-segment nucleotide sequences from selected Iranian CCHFV strains complemented with representative strains from GenBank revealed similar topology as partial sequences with eight major clusters. A partial M segment phylogeny positioned the Iranian strains in either association with clade III (Asia-Africa) or clade V (Europe).
Conclusion: The phylogenetic analysis revealed subtle links between distant geographic locations, which we propose might originate either from international livestock trade or from long-distance carriage of CCHFV by infected ticks via bird migration.

 
Keyword(s): CCHFV, MOLECULAR EPIDEMIOLOGY, RT-PCR, PHYLOGENY, REASSORTANT VIRUS, IRAN
 
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