Paper Information

Journal:   HEPATITIS MONTHLY   MARCH 2016 , Volume 16 , Number 3; Page(s) 0 To 0.
 
Paper: 

CHARACTERIZATION OF OCCULT HEPATITIS B INFECTION AMONG INJECTING DRUG USERS IN TEHRAN, IRAN

 
 
Author(s):  ASLI MARYAM, KANDELOUEI TAHMINEH, RAHIMYAN KOROUSH, DAVOODBEGLOU FOAD, VAEZJALALI MARYAM*
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF MEDICINE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, VELENJAK ST, TEHRAN, IR IRAN
 
Abstract: 

Background: Hepatitis B virus (HBV) infection is a major health problem worldwide.
Objectives: The aim of this study was to investigate the frequency of occult hepatitis B infection (OBI) and its associated risk factors, together with the molecular characterization of the virus in injecting drug users of Tehran.
PatientsandMethods: The study consisted of 229 injecting drug users. Serumsamples were collectedandtested for the presence of hepatitis B core antibody (HBcAb) and hepatitis B surface antigen (HBsAg) by an enzyme-linked immunosorbent assay (ELISA). HBV B virus DNA was extracted from the serum samples, and a fragment of the S gene was amplified using the nested polymerase chain reaction. The genotype, subgenotypes, subtype, and S gene mutation of HBV were determined by direct sequencing. A phylogenetic tree was constructed using the neighbor-joining method.
Results: Sixty-four (28%) participants were HBcAb positive, 59 cases were HBcAb positive and HBsAg negative, and 5 cases were HBsAg positive. Hepatitis B DNA was found in three HBsAg-positive cases. Thirteen of 59 (22%) individuals were hepatitis B DNA positive.
The phylogenetic tree of hepatitis B DNA showed the existence of genotype D. The only significant correlation was between sharing a syringe and OBI.
Conclusions: In comparison with the rate of HBcAb positivity reported in other Iranian studies, the rate was higher in the present study. There were a few variations, genotypes, and subtypes among the infected injecting drug users. Further investigations are needed to unravel the molecular characterization of OBI.

 
Keyword(s): INJECTING DRUG USER, HEPATITIS B, GENOTYPE, HEPATITIS B SURFACE ANTIGEN
 
References: 
 
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