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Paper Information

Journal:   GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH   2016 , Volume 9 , Number 2; Page(s) 124 To 131.
 
Paper: 

THE GENE EXPRESSION LEVEL OF IFN-γR1 AND IFN-γR2 IN A MURINE MODEL TREATED WITH TOXOPLASMA GONDII AND ITS PRODUCTS

 
 
Author(s):  KALANI HAMED, KHANAHMAD SHAHREZA HOSEIN, DARYANI AHMAD, YOUSEFI HOSEIN ALI, PESTECHIAN NADER*, MANSOURI VAHID
 
* DEPARTMENT OF PARASITOLOGY AND MYCOLOGY, SCHOOL OF MEDICINE, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Aim: To evaluate the effect of active T. gondii tachyzoites and its products on the gene expression level of IFN-gR1 and IFN-gR2 in a murine model.
Background: Many studies have shown that the parasite Toxoplasma gondii utilizes different mechanisms to inhibit the function of IFN-
g, but the parasite effect on the function of IFN-gR1 and IFN-gR2 is still unclear.
Patients and methods: Toxoplasma lysate product (TLP), excretory/secretory products (ESPs) obtained from cell free and cell culture media as well as active tachyzoites were injected separately to their respective group each consisted of 10 BALB/c mice. One control group of 10 mice received phosphate buffered saline (PBS). All of the mice were euthanized three days after the last injection and then their peritoneal leukocytes were harvested separately. The total RNA was extracted from the samples, converted to cDNA, and the gene expression level of IFN-
gR1 and IFN-gR2 was assessed in all of the treated groups relative to the control one.
Results: There was no significant difference between each of the treated groups relative to the control group concerning the gene expression level of IFN-
gR2 (P> 0.05). Furthermore, the gene expression level of IFN-gR1 in two groups of TLP (P= 0.04) and ESP obtained from cell free medium (P= 0.008) showed a significant difference relative to the control group.
Conclusion: Findings of this study revealed a new aspect of host-T. gondii interaction in that this parasite is able to down regulate IFN-
gR1 to reduce the IFN-g effects on the infected cell.

 
Keyword(s): TOXOPLASMA GONDII, GENE EXPRESSION, IFN-γR1, IFN-γR2
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

KALANI, H., & KHANAHMAD SHAHREZA, H., & DARYANI, A., & YOUSEFI, H., & PESTECHIAN, N., & MANSOURI, V. (2016). THE GENE EXPRESSION LEVEL OF IFN-γR1 AND IFN-γR2 IN A MURINE MODEL TREATED WITH TOXOPLASMA GONDII AND ITS PRODUCTS. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, 9(2), 124-131. https://www.sid.ir/en/journal/ViewPaper.aspx?id=510382



Vancouver: Copy

KALANI HAMED, KHANAHMAD SHAHREZA HOSEIN, DARYANI AHMAD, YOUSEFI HOSEIN ALI, PESTECHIAN NADER, MANSOURI VAHID. THE GENE EXPRESSION LEVEL OF IFN-γR1 AND IFN-γR2 IN A MURINE MODEL TREATED WITH TOXOPLASMA GONDII AND ITS PRODUCTS. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH. 2016 [cited 2021August04];9(2):124-131. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510382



IEEE: Copy

KALANI, H., KHANAHMAD SHAHREZA, H., DARYANI, A., YOUSEFI, H., PESTECHIAN, N., MANSOURI, V., 2016. THE GENE EXPRESSION LEVEL OF IFN-γR1 AND IFN-γR2 IN A MURINE MODEL TREATED WITH TOXOPLASMA GONDII AND ITS PRODUCTS. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, [online] 9(2), pp.124-131. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510382.



 
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