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Paper Information

Journal:   GOVARESH JOURNAL   FALL 2015 , Volume 20 , Number SUPPLEMENT; Page(s) 43 To 43.
 
Paper: 

OPTIMIZATION OF THREE DIMENSIONAL CULTURING OF THE HEPG2 CELL LINE IN FIBRIN SCAFFOLD

 
 
Author(s):  BANIHASHEMI MEHRZAD*, SAFARI AZAM, GHASEMI YOUNES
 
* PHARMACEUTICAL SCIENCES RESEARCH CENTER, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SHIRAZ, IR IRAN
 
Abstract: 

Introduction: A potential healing to enhance curative of hepatic tissue is to deliver isolated hepatic cells to the site of an injury to promote hepatic formation. An important issue within this technology is providing of an appropriate injectable system for the delivery of hepatic cells. In this regard, fibrin scaffolds showed many advantages over other scaffolds like a cell delivery vehicles in terms of biodegradation, biocompatibility and hemostasis. The aim of this study was to determine the suitable cell culture circumstances for HepG2 cell proliferation and differentiation in 3-D fibrin scaffolds by evaluating Ca++concentrations, cell numbers, and various ratios of plasma/RPMI 1640 and thickness of fibrin scaffold.
Methods: One-stage experimental design Box-Behnken design strategy was designed by Minitab 15 software (version 15, Minitab. State College, PA) with 3 factors at three levels (low, medium and high) and 27 runs for determine the effects of ratio of plasma/RPMI 1640, Ca++ concentration and thickness on formation of fibrin gelscaffold and 3-D HepG2 culture.
Results: The optimal concentrations for fibrin scaffold fabrication were found in 0.15 mol of Ca++ concentration, 1?~105 of cell number, the 1: 4 ratio of plasma/RPMI 1640 and 2.3mm thickness of fibrin gel scaffold (500?l per each well of the 24-well of cell culture plate).
Conclusion: approach provided easy handle method and also introduced inexpensive materials by using human plasma instead of purified fibrinogen to fabricate fibrin scaffold.

 
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APA: Copy

BANIHASHEMI, M., & SAFARI, A., & GHASEMI, Y. (2015). OPTIMIZATION OF THREE DIMENSIONAL CULTURING OF THE HEPG2 CELL LINE IN FIBRIN SCAFFOLD. GOVARESH JOURNAL, 20(SUPPLEMENT), 43-43. https://www.sid.ir/en/journal/ViewPaper.aspx?id=510338



Vancouver: Copy

BANIHASHEMI MEHRZAD, SAFARI AZAM, GHASEMI YOUNES. OPTIMIZATION OF THREE DIMENSIONAL CULTURING OF THE HEPG2 CELL LINE IN FIBRIN SCAFFOLD. GOVARESH JOURNAL. 2015 [cited 2021July28];20(SUPPLEMENT):43-43. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510338



IEEE: Copy

BANIHASHEMI, M., SAFARI, A., GHASEMI, Y., 2015. OPTIMIZATION OF THREE DIMENSIONAL CULTURING OF THE HEPG2 CELL LINE IN FIBRIN SCAFFOLD. GOVARESH JOURNAL, [online] 20(SUPPLEMENT), pp.43-43. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510338.



 
 
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