Paper Information

Journal:   JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN   FALL 2016 , Volume 13 (NEW) , Number 3 (54); Page(s) 1955 To 1963.
 
Paper: 

COMPARISON OF ELISA TEST WITH HISTOPATHOLOGY IN THE DIAGNOSIS OF FELINE INFECTIOUS PERITONITIS

 
 
Author(s):  SAIDI S., SAFI S.*, MASHHADI RAFII S., KABIR F.R., MORTAZAVI P.
 
* DEPARTMENT OF PATHOLOGY AND CLINICAL PATHOLOGY, FACULTY OF SPECIALIZED VETERINARY SCIENCES, SCIENCE AND RESEARCH BRANCH, ISLAMIC AZAD UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Samples from 25 cats suspected to be affected with feline infectious peritonitis (FIP) (16 cats with the signs of wet FIP and 9 cats with the signs of dry FIP) were collected from 8 clinics in the north-west Tehran from 2013 to 2015 to determine the specificity and sensitivity of the ELISA test compared to the histopathologic findings as the gold standard. ELISA test were performed on the serum and abdominal cavity samples. Statistical analyses were performed on the obtained data by ROC analysis, Youden index, Mann-Whitney U test using SPSS and R soft wares. Sensitivity, specificity, positive and negative predictive values were calculated as 100% in 13 cats with wet FIP and 7 cats with dry FIP. The area under curve (AUC) were calculated as 1, which shows the high diagnostic value of the ELISA test. The cut-off value for the ratio of ODs in the positive cats to the negative cats were determined as 3.375. Considering the acceptable sensitivity and specificity of the studied ELISA kit for the diagnosis of FIP, the kit can be used for the diagnosis of FIP alongside with the other diagnostic tests.

 
Keyword(s): CAT, FELINE INFECTIOUS PERITONITIS (FIP), ELISA, SENSITIVITY, SPECIFICITY
 
References: 
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