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Paper Information

Journal:   JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN   SUMMER 2016 , Volume 13 (NEW) , Number 2 (53); Page(s) 1885 To 1889.
 
Paper: 

OPTIMIZATION OF PCR CONDITIONS FOR CHD-W AND CHD-Z FOR SEX IDENTIFICATION IN THE CANARY

 
 
Author(s):  ETEMADI F., FATHKHANI Z., EBRAHIMI A., HOSEINI PAJOOH K.*
 
* DEPARTMENT OF BIOTECHNOLOGY, IRANIAN RESEARCH ORGANIZATION FOR SCIENCE AND TECHNOLOGY, TEHRAN, IRAN
 
Abstract: 

Bird species are considered sexually monomorphic, so in many bird species, the sex identification is very difficult especially in young birds. Since male canary is more important in warbling, sex identification of canary before puberty is important financially. Molecular genetic techniques can be use for sex identification of animals. Genes on the sex chromosomes are unique because of their sex-specific inheritance. PCR method based on chromo helicase DNA binding protein genes (CHD-Z and CHD-W) has been explained for sex identification in canary. The aim of the present study was to optimize of the PCR conditions for CHD-Z for sex identification in the canary. In this study genomic DNA was extracted from feather bulb of 24 canaries (old and young) of canary. PCR reaction was performed by a set of primers designed on the basis of the sequence of the CHD-W gene and optimized conditions was determined as follow: 94oC, 5min for initial denaturation then 35 cycle of 94oC for 60 sec, 58oC for 35sec, 72oC for 50sec, and a final 72oC for 5min. This study showed optimized PCR can be used for sex identification of canary with high accuracy. Therefore, it is the best method for this purpose.

 
Keyword(s): CANARY, SEX IDENTIFICATION, PCR
 
 
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APA: Copy

ETEMADI, F., & FATHKHANI, Z., & EBRAHIMI, A., & HOSEINI PAJOOH, K. (2016). OPTIMIZATION OF PCR CONDITIONS FOR CHD-W AND CHD-Z FOR SEX IDENTIFICATION IN THE CANARY. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 13 (NEW)(2 (53)), 1885-1889. https://www.sid.ir/en/journal/ViewPaper.aspx?id=510081



Vancouver: Copy

ETEMADI F., FATHKHANI Z., EBRAHIMI A., HOSEINI PAJOOH K.. OPTIMIZATION OF PCR CONDITIONS FOR CHD-W AND CHD-Z FOR SEX IDENTIFICATION IN THE CANARY. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN. 2016 [cited 2021July27];13 (NEW)(2 (53)):1885-1889. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510081



IEEE: Copy

ETEMADI, F., FATHKHANI, Z., EBRAHIMI, A., HOSEINI PAJOOH, K., 2016. OPTIMIZATION OF PCR CONDITIONS FOR CHD-W AND CHD-Z FOR SEX IDENTIFICATION IN THE CANARY. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, [online] 13 (NEW)(2 (53)), pp.1885-1889. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=510081.



 
 
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