Paper Information

Journal:   ARCHIVES OF IRANIAN MEDICINE   AUGUST 2016 , Volume 19 , Number 8; Page(s) 561 To 570.
 
Paper: 

DOPAMINERGIC INDUCTION OF UMBILICAL CORD MESENCHYMAL STEM CELLS BY CONDITIONED MEDIUM OF CHOROID PLEXUS EPITHELIAL CELLS REDUCES APOMORPHINE-INDUCED ROTATION IN PARKINSONIAN RATS

 
DOI: 

0161908/AIM.008

 
Author(s):  ALIAGHAEI A., GARDANEH MOSSA*, MAGHSOUDI NADER*, SALEHINEJAD PARVIN, GHARIB EHSAN
 
* NEUROSCIENCE RESEARCH CENTER (NRC), SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, EVIN, TEHRAN, IRAN
 
Abstract: 

BACKGROUND/OBJECTIVE: Degeneration of dopaminergic neurons in Parkinson’s disease (PD) implies cell replacement using potentially differentiable sources as a promising therapeutic solution. We tested the capacity of conditioned medium from choroid plexus epithelial cells (CPECs-CM) to induce the dopaminergic potential of umbilical cord matrix mesenchymal stem cells (UCMSCs).
METHODS: We isolated UCMSCs from human umbilical cord and CPECs from rat brain. Following expansion and characterization, CPECs-CM were collected, tested for expression of various growth factors, and applied to UCMSCs. Differentiation was examined and UCMSCs were injected into 6-OHDA-leasioned striatum to test their survival and function.
RESULTS: RT-PCR and immuno-staining demonstrated neuronal/dopaminergic signaling in UCMSCs induced by CPECs-CM and accelerated by addition of retinoic acid (RA) and fibroblast growth factor-2. Expression of
b-tubulin-3, Nestin and MAP2 confirmed neuronal differentiation whereas tyrosine hydroxylase, aromatic acid decarboxylase and dopamine transporter were expressed as signs of dopaminergic differentiation. Post-transplantation, the UCMSCs survived, showed reduced rate of apoptosis and led to animals’ recovery from apomorphine-induced rotations.
CONCLUSION: The combination of neurotrophic factors present in CPECs-CM and RA can synergize to maximize dopaminergic differentiation of potential cell sources including UCMSCs. Our study may have implications for PD cell replacement therapy.

 
Keyword(s): CHOROID PLEXUS, DOPAMINERGIC, MESENCHYMAL STEM CELLS, PARKINSON, UMBILICAL CORD
 
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