Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   GOVARESH JOURNAL   FALL 2015 , Volume 20 , Number SUPPLEMENT; Page(s) 0 To 0.
 
Paper: 

A NEW TOOL FOR GANGLION CELLS DIAGNOSIS IN HIRSCHSPRUNG DISEASE: THE CALRETININ MARKER ROLE

 
 
Author(s):  SAFARNEZHAD TAMESHKEL FAHIMEH, ZAMANI FARHAD, IMANZADE FARID, PANAHI MAHSHID, SOHRABI MASOUD REZA, KARBALAIE NIYA MOHAMMAD HADI, RAKHSHANI NASSER*
 
* GASTROINTESTINAL AND LIVER DISEASES RESEARCH CENTER, FIROOZGAR HOSPITAL, IRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Introduction: Hirschsprung disease (HD) is acomplex genetic disorder of the enteric nervous system (ENS), which is also often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic and/or histochemical staining of sections from suction rectal biopsies. Calretinin immunohistochemistry (IHC) may be a useful in diagnosis of Hirschsprung disease, because loss of Calretinin immunoreactive in the ganglion cells or intrinsic nerve fibers (INFs) that reported correlates specially with aganglionosis. This study showed, usefulness of immunohistochemical staining evaluation for Calretinin in rule out of Hirschsprung disease.
Methods: In this study paraffin blocks and slides were retrieved from the pathology archives of Ali Asghar hospital from 2007 to 2011 with pathology report based on the presence (14 patients) or absence (70 patients) of ganglion cells and transitional zone anatomical region (10 patients). Slides were stained with hematoxylin and eosin method to confirm the initial diagnosis was verification again. After preparing the slides, they were stained by IHC for Calretinin. Then, the results were analyzed using SPSS software.
Results: In most patients, IHC for Calretinin provided highly compatible results with hematoxylin-eosin findings in diagnosis of Hirschsprung disease. The values of specificity and accuracy between Calretinin and standard histology (H& E) that Compared by the Fisher exact test were declared Calretinin presented significantly higher specificity and accuracy values than H& E staining.
Conclusion: Calretinin IHC overcomes most of the difficulties encountered using the of histology hematoxylin-eosin. Then, IHC for Calretinin is a good ancillary method that can be used by pathologists in diagnosis of Hirschsprung disease.

 
Keyword(s): 
 
References: 
  • ندارد
 
  Yearly Visit 57
 
Latest on Blog
Enter SID Blog