Paper Information

Journal:   INTERNATIONAL JOURNAL OF HEMATOLOGY-ONCOLOGY AND STEM CELL RESEARCH (IJHOSCR)   JANUARY 2015 , Volume 9 , Number 1; Page(s) 1 To 4.
 
Paper: 

PRESENCE OF BOTH MESENCHYMAL AND CARCINOMATOUS FEATURES IN AN IN-VITRO MODEL OF OVARIAN CARCINOSARCOMA DERIVED FROM PATIENTS’ ASCITIC FLUID

 
 
Author(s):  SHARIF TABRIZI AHMAD, PELLICCIOTTA ILENIA, ABDULLAH AMER, VENDITTI CHARIS ANNE, SAMUELSON ROBERT, SHAHABI SHOHREH*
 
* DEPARTMENT OF OBSTETRICS, GYNECOLOGY AND REPRODUCTIVE BIOLOGY, DANBURY HOSPITAL, 24 HOSPITAL AVENUE, DANBURY, CT 06810, USA TEL: 203-739-7466
 
Abstract: 

We have refined the technique for isolating and propagating cultures of primary ovarian carcinosarcoma cells (OSCs) derived from ascites, which allowed the cells to obtain the biphasic features of carcinosarcoma in cell culture conditions (presence of both carcinoma and mesenchymal morphologic types). This protocol involves a simple yet rapid method for the growth and propagation of ascites OSC in a basal culture medium. Autologous ascitic fluid was used as source of growth factors, and minimal manipulation was involved to establish the culture. The methodology allowed for the direct application of multiple molecular, cellular, and functional analyses within a few weeks of initial cell isolation, with the further potential of retrospective analyses of archived cells and tissues.

 
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