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Paper Information

Journal:   GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH   2016 , Volume 9 , Number 3; Page(s) 174 To 179.
 
Paper: 

CREATION OF A MURINE ORTHOTOPIC HEPATOMA MODEL WITH INTRA-ABDOMINAL METASTASIS

 
 
Author(s):  HARRIS JAMIE, KAJDACSY BALLA ANDRE, CHIU BILL*
 
* DIVISION OF PEDIATRIC SURGERY, UNIVERSITY OF ILLINOIS CHICAGO, 840 S. WOOD STREET, DEPARTMENT OF SURGERY (MC 958) ROOM 416, CLINICAL SCIENCES BUILDING, CHICAGO, USA
 
Abstract: 

Aim: To create an orthotopic hepatoma model with local metastasis monitored with ultrasound could be created as a platform for testing new treatments.
Background: Hepatoma accounts for 25% of liver tumors in children with poor overall survival. Intraabdominal metastasis are present in 35% of patients at time of diagnosis. We hypothesized that an orthotopic tumor model with local metastasis could be created as a platform for testing treatment modalities and could be monitored with ultrasound.
Patients and methods: One million human hepatoma cells (Hep3B) were injected into the left lobe of the liver of immunocompromised mice. Tumor volume was monitored with high frequency-ultrasound until it reached 1,000mm3. At that time animals were sacrificed and examined for gross metastatic disease. Tumor sections were analyzed with hematoxylin and eosin (H&E) staining.
Results: Tumor formed in 8.15 mice. The tumor was detected as small as 19.59mm3 on ultrasound. Of the forming tumors, tumor size was 145
±177.93mm3 at 60 days post-injection, 665±650.39mm3 at 67 days, and reached >1000mm3 by 76.6±9.9 days. At necropsy, four mice (50%) had tumor only within the liver, four (50%) had additional tumors in omentum, pelvis and peritoneum. H&E showed tumor within the normal liver parenchyma, with multiple mitotic figures, small areas of necrosis, and hemorrhage within the tumor.
Conclusion: We have successfully established an orthotopic hepatoma murine model, with a local metastatic rate of 50%. Non-invasive tumor monitoring is feasible via ultrasound.

 
Keyword(s): HEPATOMA, ULTRASOUND, METASTATIC, MOUSE, ORTHOTOPIC
 
 
References: 
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Citations: 
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+ Click to Cite.
APA: Copy

HARRIS, J., & KAJDACSY BALLA, A., & CHIU, B. (2016). CREATION OF A MURINE ORTHOTOPIC HEPATOMA MODEL WITH INTRA-ABDOMINAL METASTASIS. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, 9(3), 174-179. https://www.sid.ir/en/journal/ViewPaper.aspx?id=509996



Vancouver: Copy

HARRIS JAMIE, KAJDACSY BALLA ANDRE, CHIU BILL. CREATION OF A MURINE ORTHOTOPIC HEPATOMA MODEL WITH INTRA-ABDOMINAL METASTASIS. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH. 2016 [cited 2021July25];9(3):174-179. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509996



IEEE: Copy

HARRIS, J., KAJDACSY BALLA, A., CHIU, B., 2016. CREATION OF A MURINE ORTHOTOPIC HEPATOMA MODEL WITH INTRA-ABDOMINAL METASTASIS. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, [online] 9(3), pp.174-179. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509996.



 
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