Paper Information

Journal:   GOVARESH JOURNAL   FALL 2015 , Volume 20 , Number SUPPLEMENT; Page(s) 33 To 33.
 
Paper: 

EFFECTS OF GEL AND AQUEOUS EXTRACT OF MELILOTUS OFFICINALIS L. IN INDUCED ULCERATIVE COLITIS IN RAT: AN ANTIOXIDANT AND ANTI-INFLAMMATORY EVALUATION

 
 
Author(s):  SAFARPOUR ALI REZA, KAVIYANI FATEMEH, SEPEHRIMANESH MASOOD*, AHMADI NASROLLAH, KOOHI HOSSEINABADI OMID, TANIDEH NADER, SHOWRAKI NAJMEH
 
* GASTROENTEROHEPATOLOGY RESEARCH CENTER, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SHIRAZ, I.R. IRAN
 
Abstract: 

Introduction: Inflammatory bowel diseases (IBD), which consist of two types, Crohn's disease (CD) and ulcerative colitis (UC), are chronic inflammatory disease of the gastrointestinal tract. Indeed, UCis a relapsing non-transmural inflammatory disease restricted to the colon and is one of theidiopathic disease of IBD.
Methods: The Melilotus officinalis aqueous extraction was produced and high performanceliquid chromatography and ferric reducing antioxidant power (FRAP) assay were performed onaqueous extract to identify the compounds and antioxidant activity. Seventy adultmale rats were used and UC was induced using 3% acetic acid solution. They received differentdaily dose of M. officinalis in two forms of orally (500 and1000 mg/kg) and gel extract (10%and 20%). On the 7th days, the colon tissues were examined macroscopic and histopathologically.
In addition, oxidative stress markers were evaluated and compared between all groups.
Results: We found that 1.94 mg of gallic acid, 19.8 mg of catechin, 4.38 mg of caffeic acid, 0.17 mg of chlorogenic acid, 0.23 mg of quercetin, 109.78 mg of cinnamic acid, 57.8 mg of coumarin and 5.04 mg of P-coumaric acid in 1 mg of the the flower powder.
The FRAP value for the extract was 2.91
± 0.14 ?M/g.
There are significant differences between the groupof rats which received the gel or aqueous extract of the flower compared to the negative control group using normal saline and the base gel and they were close with the positive control group using the asacol, regarding the pathologic, malondialdehyde, and weight improvements.
Conclusion: Our findings are interesting for the strategy of selecting the medical plant as treatment for UC. The antioxidant and anti-inflammatory effects of M. officinalis L. extract seem to be clear, especially by identifying catechin and cinnamic acid as the most phenolic acid and comarin constituents, but its molecular anti-inflammatory mechanisms have not yet been fully investigated. Therefore, performing further studies for identifying this mechanism in cell culture and also animal and human studies are highly recommended.

 
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