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Paper Information

Journal:   IRANIAN JOURNAL OF MICROBIOLOGY   APRIL 2016 , Volume 8 , Number 2; Page(s) 153 To 158.
 
Paper: 

ASSOCIATION OF HHV-6 WITH HODGKIN AND NON HODGKIN LYMPHOMA

 
 
Author(s):  KIANI HADIS, MAKVANDI MANOOCHEHR*, SAMARBAFZADEH ALIREZA, TEIMOORI ALI, NISI NILOOFAR, MEHRAVARAN HAMIDE, RADMEHR HASHEM, HOSSEINI ZEINAB, HAGHI AZADEH, SHAHANI TORAN, VARNASERI MEHRAN, RANJBARI NASTARAN
 
* VIROLOGY DEPARTMENT, SCHOOL OF MEDICINE, AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES, AHVAZ, IRAN
 
Abstract: 

Background and Objectives: Human Herpes 6 virus (HHV-6) could remain latent and chronic in the host cells after primary infection. HHV-6 genome encodes certain transactivation proteins which may results in development of malignant lymphoma. The association of human herpes six virus (HHV-6) infection and Hodgkin and Non-Hodgkin lymphomas is strongly supported by epidemiological studies. The aim of this study was to determine the prevalence of HHV-6 among the patients with Hodgkin, Non- Hodgkin`s lymphoma.
Materials and Methods: Overall 44 blocks of formalin-fixed, paraffin-embedded of the patients including 22(50%) Hodgkin and 22(50%) Non-Hodgkin Lymphoma were collected. Initially the section of 5?m-thickness were prepared from the formalin-fixed, paraffin-embedded tissue blocks. Then the deparaphinazation was carried out for each sample. The DNA was extracted, followed by nested PCR for detection of HHV-6. Based on PCR product size and sequencing, the HHV-6 A or B subtypes were characterized.
Results: 12.22 (54.54%) cases of Hodgkin and 8.22 (36.36%) Non-Hodgkin’s lymphoma were shown as positive for HHV-6. Out of 12 positive HHV-6 in Hodgkin lymphoma, 10 patients (45.45%) belonged to variant A while 2 cases (9.09%) were found positive for both HHV-6A and HHV-6B. All the Non Hodgkin samples (n=8, 36.36%) showed positive for HHV-6 variant A.
Conclusion: High prevalence of HHV-6 was found among the patients with Hodgkin and Non-Hodgkin’s lymphoma. Two patients with Hodgkin lymphoma had mixed HHV-6A and HHV-6B infections. It is recommended patients with Hodgkin and Non-Hodgkin should be screened for HHV-6 detection before chemotherapy.

 
Keyword(s): NESTED PCR, HUMAN HERPES VIRUS 6, HODGKIN LYMPHOMA, NON-HODGKIN LYMPHOMA
 
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