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Paper Information

Journal:   IRANIAN JOURNAL OF MICROBIOLOGY   APRIL 2016 , Volume 8 , Number 2; Page(s) 132 To 138.
 
Paper: 

EVALUATION OF THE EUKARYOTIC EXPRESSION OF MTB32C-HBHA FUSION GENE OF MYCOBACTERIUM TUBERCULOSIS IN HEPATOCARCINOMA CELL LINE

 
 
Author(s):  TEIMOURPOUR ROGHAYEH, ZARE HOSNA, RAJABNIA RAMAZAN, YAHYAPOUR YOUSEF, MESHKAT ZAHRA*
 
* ANTIMICROBIAL RESISTANCE RESEARCH CENTER, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, IR IRAN
 
Abstract: 

Background and Objectives: HBHA and Mtb32C have been isolated from culture supernatants of Mycobacterium tuberculosis (M. tuberculosis) and Mycobacterium bovis (M. bovis) and their immunogenicity previously studies have been confirmed. In this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (Mtb32C-HBHA), in producing new chimeric protein under the in-vitro condition was examined.
Materials and Methods: In present study Huh7.5 cells was transfected with Mtb32C-HBHA -pCDNA3.1+ recombinant vector using the calcium phosphate method and expression of chimeric protein was assessed by RT-PCR and Western blot methods.
Results: Results of RT-PCR and Western blot showed expression of 35.5 KD recombinant protein (Mtb32C-HBHA) in this cell line.
Conclusion: The constructed vector can produce two highly immunogenic antigens that fusion of them to gather makes chimeric antigen with new traits. Other attempts are needed to evaluate specific properties of this new antigen such as molecular conformation modeling and immunologic characteristics in future studies.

 
Keyword(s): MYCOBACTERIUM TUBERCULOSIS, HBHA, MTB32C, HUH7.5 CELLS
 
 
References: 
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Citations: 
 
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APA: Copy

TEIMOURPOUR, R., & ZARE, H., & RAJABNIA, R., & YAHYAPOUR, Y., & MESHKAT, Z. (2016). EVALUATION OF THE EUKARYOTIC EXPRESSION OF MTB32C-HBHA FUSION GENE OF MYCOBACTERIUM TUBERCULOSIS IN HEPATOCARCINOMA CELL LINE. IRANIAN JOURNAL OF MICROBIOLOGY, 8(2), 132-138. https://www.sid.ir/en/journal/ViewPaper.aspx?id=509942



Vancouver: Copy

TEIMOURPOUR ROGHAYEH, ZARE HOSNA, RAJABNIA RAMAZAN, YAHYAPOUR YOUSEF, MESHKAT ZAHRA. EVALUATION OF THE EUKARYOTIC EXPRESSION OF MTB32C-HBHA FUSION GENE OF MYCOBACTERIUM TUBERCULOSIS IN HEPATOCARCINOMA CELL LINE. IRANIAN JOURNAL OF MICROBIOLOGY. 2016 [cited 2021July31];8(2):132-138. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509942



IEEE: Copy

TEIMOURPOUR, R., ZARE, H., RAJABNIA, R., YAHYAPOUR, Y., MESHKAT, Z., 2016. EVALUATION OF THE EUKARYOTIC EXPRESSION OF MTB32C-HBHA FUSION GENE OF MYCOBACTERIUM TUBERCULOSIS IN HEPATOCARCINOMA CELL LINE. IRANIAN JOURNAL OF MICROBIOLOGY, [online] 8(2), pp.132-138. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509942.



 
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