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Paper Information

Journal:   JOURNAL OF MARINE SCIENCES AND TECHNOLOGY   FALL 2012 , Volume 11 , Number 3; Page(s) 64 To 71.
 
Paper: 

GENETIC DIVERSITY OF MUDSKIPPER (PERIOPHTHALMUS WALTONI) USING RAPD MARKERS IN THE PERSIAN GULF

 
 
Author(s):  YAISH BACHARI SOAD, ZOLGHARNEIN HOSSEIN*, MOHAMMADI MEHDI, SALARI ALIABADI MOHAMMAD ALI, GHASEMI SEIED AHMAD
 
* DEPARTMENT OF MARINE BIOLOGY, FACULTY OF MARINE SCIENCE, KHORRAMSHAHR UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY, KHORRAMSHAHR, IRAN
 
Abstract: 

Sixty nine specimens of Mudskipper, Periophthalmus waltoni, were collected from Hendijan, Khore Zangi and Delvar areas for genetic study. Fins were fixed in Ethanol alcohol (96%) and transferred to Biotechnology laboratory, Persian Golf Research and Studies Center in Bushehr. Total genomic DNA was extracted by proteinase-K digestion, phenol: chloroform: isoamyl alcohol extraction and ethanol precipitation. The quality and quantity of DNA was assessed using 1% agarose gel electrophoresis and spectrophotomeric method. Polymerase chain reactions (PCR) were conducted on the target DNA using 6 primers RAPD. PCR products were separated by electrophoresis on polyacrylamid gels (8%) that were stained using silver nitrate. Allele frequency, the effective and real allele, expected and observed heterozygosis, genetic diversity, Shannon Information index, Gst and gene based on analysis of molecular variance (AMOV A) were calculated using the GENALEX and Pop Gene software. The average of Shannon index for Hendijan, Khore zangi and Delvar was respectively 0.3887, 0.2860 and 0.3509. Highest of Gst observed between Hendijan and Khore zangi (0.260) and lowest between Khore zangi and Delvar (0.195). Highest gene flow (Nm) was observed between Delvar and Khore zangi (1.033) and the lowest between Hendijan and Khore zangi (0.687). Highest of genetic identity was observed between Delvar and Khore zangi (0.924) and the lowest between Hendijan and Khore zangi community (0.833). This study showed that the three mudskipper communities used inthis study were separate and belonged to two clusters.

 
Keyword(s): MUDSKIPPER, PERIOPHTHALMUS WALTONI, RAPD-PCR, PERSIAN GULF, GENETIC DISTANCE AND IDENTIFY
 
References: 
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