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Paper Information

Journal:   JOURNAL OF DIABETES AND METABOLIC DISORDERS   FEBRUARY-MARCH 2016 , Volume 15 , Number 3; Page(s) 183 To 191.
 
Paper: 

SERUM CYTOKERATIN-18 FRAGMENT LEVELS, PARAOXONASE ACTIVITY AND LIPID PROFILE OF NON-ALCOHOLIC FATTY LIVER IN IRAN

 
 
Author(s):  TEIMOURI NAHID, NAYERI HASHEM*
 
* BASIG ST., AZAD UNIVERSITY BLU , FALAVARJAN, ESFAHAN, ISLAMIC AZAD UNIVERSITY, POSTAL CODE: 84515155
 
Abstract: 

Background: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease is increasing in adults and children worldwide. Obesity, insulin resistance or diabetes type II, hyperlipidemia and hypertriglyceridemia plays a major role in the epidemiology of this disease. Cytokeratin 18 (CK-18) the major intermediate filament protein in the liver is a marker of increased hepatocyte apoptosis.
The aim of this study was todeterminate CK-18 level as a marker of hepatocyte apoptosis and paraoxonase as a biochemical marker for lipid peroxidation.
Methods: This case–control study was done on 51 subjects with confirmed NAFLD by ultrasound and 30 healthy individuals. CK-18 is proposed as a biomarker alternative cell death. The serum was used for measurement of the apoptosis-associated neo-epitope in the C-terminal domain of CK-18 by the M30-Apoptosense ELISA kit. The M30 detection antibody recognizes a neo-epitope mapped to positions 387 to 396 of CK18, so called CK18-Asp396 that is only revealed after caspase cleavage of the protein and is postulated as a selective biomarker of apoptosis. Serum PON1 activity was assayed using a synthetic substrate. Paraoxon substrate (diethyl-p nitrophenylphosphate), was deliberated using the increase of absorbance at 412 nm at 37?C.
Results: There were significant differences regarding serum cytokeratin 18 (p=0.005), paraoxonase activity (p=0.03), triglycerides (p=0.04) and low-density lipoprotein (p=0.04) between NAFLD and healthy subjects.
Between CK-18 and paraoxonase with the early stages of fatty liver disease are associated.
Conclusion: This study suggests that serum levels of cytokeratin 18 can be useful in predicting nonalcoholic fatty liver disease. Paraoxonase activity (PON1) should be considered a biochemical marker of lipid peroxidation and the need for follow-up in patients with NAFLD.

 
Keyword(s): NON-ALCOHOLIC FATTY LIVER DISEASE (NAFLD), NON-ALCOHOLIC STEATOHEPATITIS (NASH), CYTOKERATIN 18 (CK-18), PARAOXONASES
 
 
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APA: Copy

TEIMOURI, N., & NAYERI, H. (2016). SERUM CYTOKERATIN-18 FRAGMENT LEVELS, PARAOXONASE ACTIVITY AND LIPID PROFILE OF NON-ALCOHOLIC FATTY LIVER IN IRAN. JOURNAL OF DIABETES AND METABOLIC DISORDERS, 15(3), 183-191. https://www.sid.ir/en/journal/ViewPaper.aspx?id=509416



Vancouver: Copy

TEIMOURI NAHID, NAYERI HASHEM. SERUM CYTOKERATIN-18 FRAGMENT LEVELS, PARAOXONASE ACTIVITY AND LIPID PROFILE OF NON-ALCOHOLIC FATTY LIVER IN IRAN. JOURNAL OF DIABETES AND METABOLIC DISORDERS. 2016 [cited 2021May16];15(3):183-191. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509416



IEEE: Copy

TEIMOURI, N., NAYERI, H., 2016. SERUM CYTOKERATIN-18 FRAGMENT LEVELS, PARAOXONASE ACTIVITY AND LIPID PROFILE OF NON-ALCOHOLIC FATTY LIVER IN IRAN. JOURNAL OF DIABETES AND METABOLIC DISORDERS, [online] 15(3), pp.183-191. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509416.



 
 
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