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Paper Information

Journal:   IRANIAN JOURNAL OF NUCLEAR MEDICINE   SUMMER 2015 , Volume 23 , Number 2 (SERIAL NUMBER 44); Page(s) 73 To 81.
 
Paper: 

SYNTHESIS AND EXPERIMENTAL STUDY OF NORFLOXACIN LABELED WITH TECHNECIUM- 99M AS A POTENTIAL AGENT FOR INFECTION IMAGING

 
 
Author(s):  SAZONOVA SVETLANA IVANOVNA*, LISHMANOV YURIY BORISOVICH, VARLAMOVA NATALIA VALERIEVNA, SKURIDIN VIKTOR SERGEEVICH, ILUSHENKOVA YULIA NIKOLAYEVNA, KARPOVA MARIA ROSTISLAVOVNA, NESTEROV YEVGENIY ALEXANDROVICH
 
* RESEARCH INSTITUTE OF CARDIOLOGY OF THE SIBERIAN BRANCH OF THE RUSSIAN ACADEMY OF MEDICAL SCIENCES, TOMSK, RUSSIA
 
Abstract: 

Introduction: Differentiation between infection and sterile inflammation is one of the most difficult medical problems and is relevant in many clinical situations. Scintigraphy with radiolabeled antibiotics, ?specially labeled fluoroquinolones, is a promising tool for diagnosing bacterial inflammation. We have attempted to synthesize 99mTc-norfloxacin and studied its properties in experiment.
Methods: In present study we labeled norfloxacin with 99mTc, determined its radiochemical purity by thin-layer chromatography procedure, compared the bactericidal activity of 99mTc-norfloxacin and unlabelled norfloxacin by disk diffusion, performed in vitro binding assays and evaluated the feasibility of 99mTc-norfloxacin to image soft-tissue infections in rat modal of thigh muscle infection. Scintigraphy was performed at 30 min, 90 min and 18 h post injection. Abscess –to–normal-site ratios were calculated.
Results: In this study 99mTc-norfloxacin with high radiochemical purity and low colloid content was synthesized. Disk diffusion method showed that 99mTc-norfloxacin retains bactericidal properties of unlabelled norfloxacin. In vitro binding studies demonstrated small degree of the tracer binding with S. aureus and ability of 99mTc-norfloxacin to bind with killed as well as alive bacteria. Uptake of the tracer was clearly visible in infected muscles and not visible in sterile inflammed muscles.
Conclusion: The 99mTc-norfloxacin retains bactericidal properties of unlabelled norfloxacin. The tracer demonstrated small degree of S. aureus binding both with killed as well as alive bacteria. Studies which were performed in vivo in rats with a model of thigh muscle infection showed good ability of the radiopharmaceutical to image the infection.

 
Keyword(s): NORFLOXACIN, TECHNECIUM-99M, INFECTION, INFLAMMATION, SCINTIGRAPHY
 
 
References: 
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APA: Copy

SAZONOVA, S., & LISHMANOV, Y., & VARLAMOVA, N., & SKURIDIN, V., & ILUSHENKOVA, Y., & KARPOVA, M., & NESTEROV, Y. (2015). SYNTHESIS AND EXPERIMENTAL STUDY OF NORFLOXACIN LABELED WITH TECHNECIUM- 99M AS A POTENTIAL AGENT FOR INFECTION IMAGING. IRANIAN JOURNAL OF NUCLEAR MEDICINE, 23(2 (SERIAL NUMBER 44)), 73-81. https://www.sid.ir/en/journal/ViewPaper.aspx?id=509368



Vancouver: Copy

SAZONOVA SVETLANA IVANOVNA, LISHMANOV YURIY BORISOVICH, VARLAMOVA NATALIA VALERIEVNA, SKURIDIN VIKTOR SERGEEVICH, ILUSHENKOVA YULIA NIKOLAYEVNA, KARPOVA MARIA ROSTISLAVOVNA, NESTEROV YEVGENIY ALEXANDROVICH. SYNTHESIS AND EXPERIMENTAL STUDY OF NORFLOXACIN LABELED WITH TECHNECIUM- 99M AS A POTENTIAL AGENT FOR INFECTION IMAGING. IRANIAN JOURNAL OF NUCLEAR MEDICINE. 2015 [cited 2021May14];23(2 (SERIAL NUMBER 44)):73-81. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509368



IEEE: Copy

SAZONOVA, S., LISHMANOV, Y., VARLAMOVA, N., SKURIDIN, V., ILUSHENKOVA, Y., KARPOVA, M., NESTEROV, Y., 2015. SYNTHESIS AND EXPERIMENTAL STUDY OF NORFLOXACIN LABELED WITH TECHNECIUM- 99M AS A POTENTIAL AGENT FOR INFECTION IMAGING. IRANIAN JOURNAL OF NUCLEAR MEDICINE, [online] 23(2 (SERIAL NUMBER 44)), pp.73-81. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509368.



 
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