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Paper Information

Journal:   BIOLOGICAL JOURNAL OF MICROORGANISM   WINTER 2016 , Volume 4 , Number 16; Page(s) 77 To 86.
 
Paper: 

OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350

 
 
Author(s):  SAMADLOUIE HAMID REZA*, KARIMIROOZBAHANI MARYAM, KABOOSI HAMI, FARROKHI NASER
 
* SHAHROOD UNIVERSITY, IRAN
 
Abstract: 

Introduction: Production of an endogenous ?-amylase from Bacillus amyloliquefaciens ATCC 23350 was studied and enhanced.
Materials and methods: Protein and carbon sources were analyzed for free and immobilized bacterial cells and number of beads was considered for immobilized cells via one factor at a time methodfor?-amylase production by Bacillus amyloliquefaciens. Subsequently, optimization condition was employed solely for immobilized bacterial cells by response surface methodology (RSM).
Results: Peptone and rice starch showed to improve the ?-amylase production in immobilized Bacillus cells. RSM generated a mathematical model explaining the optimum concentration of the efficient nutrients (139.35 g/l of rice starch and 80.00 g/l of peptone) leading to an optimum amylase production (205 U/ml).
Discussion and conclusion: The statistical advance displayed significant outcomes to optimize the process parameters for maximal ?-amylase production using Bacillus amyloliquefaciens and gave permission to rapid screening of variables. RSM led to find out an immense improvement in enzyme activity (more than 90%: from 25 to 225 U/ml) for the first time.

 
Keyword(s): BACILLUS AMYLOLIQUEFACIENS, ENZYME PRODUCTION, FERMENTATION, IMMOBILIZATION, RESPONSE SURFACE METHODOLOGY
 
 
References: 
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Click to Cite.
APA: Copy

SAMADLOUIE, H., & KARIMIROOZBAHANI, M., & KABOOSI, H., & FARROKHI, N. (2016). OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350. BIOLOGICAL JOURNAL OF MICROORGANISM, 4(16), 77-86. https://www.sid.ir/en/journal/ViewPaper.aspx?id=509339



Vancouver: Copy

SAMADLOUIE HAMID REZA, KARIMIROOZBAHANI MARYAM, KABOOSI HAMI, FARROKHI NASER. OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350. BIOLOGICAL JOURNAL OF MICROORGANISM. 2016 [cited 2021May12];4(16):77-86. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509339



IEEE: Copy

SAMADLOUIE, H., KARIMIROOZBAHANI, M., KABOOSI, H., FARROKHI, N., 2016. OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350. BIOLOGICAL JOURNAL OF MICROORGANISM, [online] 4(16), pp.77-86. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509339.



 
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