Paper Information

Journal:   NOVA BIOLOGICA REPERTA   SPRING 2016 , Volume 3 , Number 1; Page(s) 15 To 23.
 
Paper: 

ASSOCIATION BETWEEN GENE POLYMORPHISM RS11614913 IN MICRORNA196A2, WITH NON-SMALL CELL LUNG CANCER RISK IN THE POPULATION OF SOUTHERN IRAN

 
DOI: 

10.21859/acadpub.nbr.3.1.15

 
Author(s):  PISHVA PARVIN, TAHMASEB MOHAMMAD*, GHADERI ABBAS
 
* DEPARTMENT OF CELL AND MOLECULAR BIOLOGY, FACULTY OF BIOLOGICAL SCIENCES, KHARAZMI UNIVERSITY, IRAN
 
Abstract: 

Lung cancer is the fifth most common cancer in Iran, with rates of 4.7 to 9.2 per 100 thousand populations. Despite the low incidence, survival is not promising. Using biological markers could lead to longer survival rate in patients due to early diagnosis. In this study, we evaluated the association of SNP rs11614913 in mir-196a2 with risk of non-small cell lung cancer (NSCLC) in population of southern Iran. SNP rs11614913 in miR-196a2 was assayed in 95 lung cancer patients and 100 controls. The extracted DNA from peripheral blood was amplified using specific primers followed by TaaI digestion. Based on the nucleotide base in this SNP (T or C) two different patterns were produced. In case of T, two bands (187bp and 196bp) and in case of C just one band (383bp) was generated. Analysis of electrophoretic pattern of digestion products revealed that the frequencies of C allele for SNP rs11614913 miR-196a2 were 0.695 in patients and 0.76 in controls. The statistical analysis indicates the presence of Hardy-Weinberg equilibrium between the two alleles in the population studied (p>0.05). Based on the results in this study, no significant association between SNP rs11614913 and susceptibility to NSCLC was found. However, male participants who possess TC/TT genotypes showed high risk for NSCLC compared to those possessed CC genotypes.

 
Keyword(s): MICRO RNA, NON-SMALL CELL LUNG CANCER, SNP
 
References: 
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