Paper Information

Journal:   BIOLOGICAL JOURNAL OF MICROORGANISM   WINTER 2016 , Volume 4 , Number 16; Page(s) 55 To 64.
 
Paper: 

BIODEGRADABLE PLASTICS FROM SINO RHIZOBIUM MELILOTI AS PLASTICS COMPATIBLE WITH THE ENVIRONMENT AND HUMAN HEALTH

 
 
Author(s):  HASHEMI BEIDOKHTI MEHRDAD, NAKHAEI MOGHADDAM MAHBOOBEH*
 
* DEPARTMENT OF BIOLOGY, MASHHAD BRANCH, ISLAMIC AZAD UNIVERSITY, MASHHAD, IRAN
 
Abstract: 

Introduction: Polyhydroxyalkanoates (PHAs) are natural polyesters and biodegradable plastics that are stored as intracellular inclusion bodies by a great variety of bacteria. The aim of this study was to extract polyhydroxyalkanoate from native Sino rhizobium meliloti in Iran.
Materials and methods: Sino rhizobium meliloti isolates were collected from roots of alfalfa plants and were identified by Gram staining, biochemical experiments and amplification of 1500 bp fragment of 16Sr DNA gene. PHA granules were detected by microscopic examination. PHA production was evaluated in nutrient deficient medium and its amount was determined by conversion of PHA into crotonic acid by sulphuric acid treatment. The effect of various temperatures, agitation rate and carbon source (sucrose, mannitol, and maltose) were evaluated on dry cell weight and polyhydroxybutyrate (PHB) production.
Results: The maximum amount of polymer production (43.10%) was seen in basal mineral medium at 29°C, pH
~7 and 215 revolutions per minute (rpm). The results of this research showed that the S5 isolate was capable to produce maximum poly3- hydroxybutyrate. The produced polymer was analyzed for its purity by GC- mass (gas chromatography- mass spectroscopy) and confirmed to be PHB compared with the standard polymer.
Discussion and conclusion: Native strains of Sino rhizobium can be used in the production of biodegradable plastics and the results of present study showed that S. meliloti S5 was capable to produce maximum PHB at 29°C, agitation rate of 215 rpm, and pH
~7.

 
Keyword(s): POLYHYDROXYALKANOATE, SINO RHIZOBIUM MELILOTI, BIODEGRADABLE PLASTICS, GC- MASS
 
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