Paper Information

Journal:   BIOLOGICAL JOURNAL OF MICROORGANISM   WINTER 2016 , Volume 4 , Number 16; Page(s) 33 To 42.
 
Paper: 

REPORT OF NOCARDIA SPECIES ISOLATED FROM SOIL BY 16S RRNA GENE SEQUENCING METHOD IN ISFAHAN, IRAN

 
 
Author(s):  BOURBOUR SAMANEH, FAGHRI JAMSHID*, MOGHIM SHARAERH
 
* ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, IRAN
 
Abstract: 

Introduction: The genus Nocardia belongs to aerobic Actinomycetes. They are a large group of soil-dwelling bacteria that are distributed worldwide. Using various key conventional and molecular diagnostic tests, several Nocardia isolates were identified, characterized and distinguished.
Materials and methods: In our study, soil isolation method was Slip-buried type and DNA extraction was obtained through Microwave oven method and Nocardia asteroids DSM 43757-type strain as standard was tested to approve the above method. Following this study, Polymerase Chain Reaction was carried out using universal primers.
Results: Phenotypic and molecular data analysis, particularly 16S rRNA gene sequencing, provided evidence on Nocardia cyriacigeorgica KC577151, Nocardia asteroides KC577152, Nocardia cummidelens KC577153, Nocardia asteroides KC577154, Nocardia asteroides KC577155, Nocardia coubleae KC577156 involvement in Iran soil in Isfahan and these isolates were eventually registered in NCBI gene bank.
Discussion and conclusion: In this research, six Nocardia species were isolated and identified some of which isolated species were novel in Iran soil in Isfahan, at the time of isolation and detection. To identify more species of Nocardia in subsequent studies, proliferation and sequencing of other genes of the bacteria such as hsp65, ITS, secA, rpoB and sod can be applied.

 
Keyword(s): NOVEL NOCARDIA SPECIES, 16S RRNA SEQUENCING, SOIL SAMPLES, ISFAHAN, IRAN
 
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