Paper Information

Journal:   PAJOUHESH DAR PEZESHKI   2015 , Volume 39 , Number 4; Page(s) 156 To 162.
 
Paper: 

HUMAN-BONE MARROW MESENCHYMAL STEM CELLS-CONDITIONED MEDIUM AND LOW-LEVEL LASER THERAPY INCREASE TROPHIC FACTOR EXPRESSION IN DIABETIC FIBROBLASTS

 
 
Author(s):  PIRYAEI ABBAS*, HENDUDARI FARZANE, BAYAT MOHAMMAD, HASSANI SEYEDEH NAFISEH
 
* DEPARTMENT OF BIOLOGY AND ANATOMICAL SCIENCES, SCHOOL OF MEDICINE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: Chronic wounds of diabetic patients, which resulted from some impairments including disruption in production and secretion of effective factors in the process of healing is highly regarded. In recent years, many studies have been reported the positive impact of low level laser therapy (LLLT) and mesenchymal stem cells (MSCs) and their conditioned media (CM) in diabetic wound healing. In the present study we evaluated the combined effect of LLLT and human-bone marrow (h-BM) MSCs-CM on the expression of an inflammatory factor and a growth factor, both involved in wound healing, in cultured fibroblasts at high glucose condition.
Materials and Methods: In this experimental study human dermal fibroblasts (HDFs) were cultured at culture media with normal and high glucose (as diabetic model) concentrations. Then the diabetic fibroblasts were treated by a laser beam with 0.5J/cm2 and h-BMMSCs-CM, separately and in combination with each other. Finally, the gene expression of bFGF and TGF-
b2 were evaluated, using RT-PCR technique.
Result: The expression of bFGF, significantly and the expression of TGF-
b2, non-significantly were increased in all three treated diabetic groups, compared to the untreated diabetic group. Despite the relative expression of bFGF in diabetic group was 0.47±0.11. It significantly (p<0.05) rose to 1.23±0.35 in laser treated group, 0.98±0.028 in CM treated group and 1.37±0.2 in Lasr+CM treated group.
Conclusion: Apparently, by their synergistically effects, using the LLLT and the h-BMMSCs-CM together, positively affect the expression of tropic factor involved in diabetic wound healing.

 
Keyword(s): LOW LEVEL LASER THERAPY, MESENCHYMAL STEM CELLS, CONDITIONED MEDIUM, WOUND HEALING, DIABETES
 
References: 
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