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Paper Information

Journal:   JOURNAL OF FISHERIES SCIENCE AND TECHNOLOGY (JFST)   SPRING 2016 , Volume 5 , Number 1; Page(s) 69 To 82.
 
Paper: 

OPTIMIZATION OF PERSIAN GULF CUTTLEFISH (SEPIA PHARAONIS) MUSCLE HYDROLYSATE PRODUCTION BY RSM

 
 
Author(s):  HAMZEH ALI, REZAEI MASOUD*, KHODABANDEH SABER, MOTAMEDZADEGAN ALI, NORUZINIA MEHRDAD
 
* DEPT. OF SEAFOOD PROCESSING, FACULTY OF MARIN SCIENCES, TARBIAT MODARES UNIVERSITY, NOOR, IRAN
 
Abstract: 

The response surface methodology was employed to optimize the effects of pH, temperature (oC), time (min) and the ratio of enzyme to substrate (% of substrate) on the hydrolysis process of cuttlefish muscle by alcalase. Central composite rotatable design with 5 levels and 4 factors and a=2 was used for the optimization of the process to gain the highest degree of hydrolysis. pH, temperature, time, enzyme concentration, interaction of temperature-enzyme concentration, square of pH, temperature, time and enzyme concentration had significant effects on the process. The R2=0.95, lack of fit<0.05 and adeq-Precision of 14.16 for the model showed that the model could explain the variability within the range of values. The optimum condition for 42.0117 % of degree of hydrolysis was determined by Design Expert as pH 8.19, temperature 50.23, time 129.62 and enzyme2.15%.

 
Keyword(s): CUTTLEFISH, HYDROLYSATE, RSM, ALCALASE, CCRD
 
References: 
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