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Paper Information

Journal:   PAJOUHESH DAR PEZESHKI   2015 , Volume 39 , Number 2; Page(s) 87 To 91.
 
Paper: 

EVALUATION OF THE RESULTS OF LYMPHOCYTE TRANSFORMATION TEST IN PATIENTS WITH HYPERSENSITIVITY REACTIONS FOLLOWING PHENYTOIN USAGE AND CONTROL GROUP

 
 
Author(s):  KARAMI ZAHRA, MESDAGHI MEHRNAZ*, CHAVOSHZADEH ZAHRA, KARIMZADEH PARVANEH, MANSOURI MAHBOUBEH, SHEKARRIZ REZA
 
* DEPARTMENT OF IMMUNOLOGY, SCHOOL OF MEDICINE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: Administration of phenytoin can been associated with severe adverse reactions such as hypersensitivity reactions. Lymphocyte transformation test (LTT) can be a useful method for determination of the drug, which has caused hypersensitivity reaction. This study was done to evaluate the results of lymphocyte transformation test in patients with delayed hypersensitivity reactions following phenytoin administration and control group.
Methods: In a case-control study, four patients with hypersensitivity reactions following administration of phenytoin and ten patients, who had used phenytoin without hypersensitivity reactions, were included. Peripheral blood mononuclear cells were isolated. The cells were stimulated with Phenytoin, PHA (Phytohaemagglutinin) as a mitogen and candida as an antigen. Lymphocyte proliferation was measured using Brdu proliferation assay kit (Roche, Germany). The stimulation index was calculated by dividing OD of stimulated to unstimulated cells.
Stimulation index more than 2 was considered as positive. The results in case and control groups were compared, using Fisher’s exact test.
Results: Out of 4 patients in the test group, 3 had positive LTT results and one had negative test result. Among patients in control group, none of them had positive LTT result. This difference was statistically significant (p=0.002). The mean time between development of drug reactions and perform the LTT was 16±6.9 months in the test group with positive result and 38 months in test group with negative test result. This difference was not statistically significant (p=0.174).

 
Keyword(s): LTT CAN BE A HELPFUL METHOD IN DIAGNOSIS OF THE DRUG THAT HAS CAUSED HYPERSENSITIVITY REACTION
 
 
References: 
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Click to Cite.
APA: Copy

KARAMI, Z., & MESDAGHI, M., & CHAVOSHZADEH, Z., & KARIMZADEH, P., & MANSOURI, M., & SHEKARRIZ, R. (2015). EVALUATION OF THE RESULTS OF LYMPHOCYTE TRANSFORMATION TEST IN PATIENTS WITH HYPERSENSITIVITY REACTIONS FOLLOWING PHENYTOIN USAGE AND CONTROL GROUP. PAJOUHESH DAR PEZESHKI, 39(2), 87-91. https://www.sid.ir/en/journal/ViewPaper.aspx?id=509017



Vancouver: Copy

KARAMI ZAHRA, MESDAGHI MEHRNAZ, CHAVOSHZADEH ZAHRA, KARIMZADEH PARVANEH, MANSOURI MAHBOUBEH, SHEKARRIZ REZA. EVALUATION OF THE RESULTS OF LYMPHOCYTE TRANSFORMATION TEST IN PATIENTS WITH HYPERSENSITIVITY REACTIONS FOLLOWING PHENYTOIN USAGE AND CONTROL GROUP. PAJOUHESH DAR PEZESHKI. 2015 [cited 2021May10];39(2):87-91. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509017



IEEE: Copy

KARAMI, Z., MESDAGHI, M., CHAVOSHZADEH, Z., KARIMZADEH, P., MANSOURI, M., SHEKARRIZ, R., 2015. EVALUATION OF THE RESULTS OF LYMPHOCYTE TRANSFORMATION TEST IN PATIENTS WITH HYPERSENSITIVITY REACTIONS FOLLOWING PHENYTOIN USAGE AND CONTROL GROUP. PAJOUHESH DAR PEZESHKI, [online] 39(2), pp.87-91. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=509017.



 
 
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