Background: This research was carried out for a by considering gene sequence and each amino acid codon in host, which express protein in order of probable vaccine production against influenza A, a major cause of mortality in a year. M2 protein is conserved and it can be a proper candidate vaccine for influenza infection.
Materials and Methods: Present research was an experimental study. The sequence of M2 protein of Iran influenza virus H1N1 strain was modified and synthesized. It was subcloned into pET22b vector recombinant protein and expressed in BL21 E-coli and confirmed with specific antibody by western blot technique.
Results: The modified M2 gene was expressed in E-coli. The concentration of it was 300?g/ml and it was confirmed by western blotting with specific antibody.
Conclusion: Influenza M2 protein with modification in sequence could be expressed in BL21 E-coli host.