Paper Information

Journal:   PAJOUHESH DAR PEZESHKI   2015 , Volume 39 , Number 2; Page(s) 69 To 73.
 
Paper: 

EVALUATION OF MODIFICATION IN M2 GENE OF INFLUENZA H1N1 VIRUS IN EXPRESSION IN BL21 E-COLI

 
 
Author(s):  JAFARINEJAD FATEMEH, BANDEHPOUR MOJGAN*, GHEFLAT SHIVA SADAT, KAZEMI BAHRAM
 
* DEPARTMENT OF BIOCHEMISTRY, SCHOOL OF ADVANCED TECHNOLOGIES IN MEDICINE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: This research was carried out for a by considering gene sequence and each amino acid codon in host, which express protein in order of probable vaccine production against influenza A, a major cause of mortality in a year. M2 protein is conserved and it can be a proper candidate vaccine for influenza infection.
Materials and Methods: Present research was an experimental study. The sequence of M2 protein of Iran influenza virus H1N1 strain was modified and synthesized. It was subcloned into pET22b vector recombinant protein and expressed in BL21 E-coli and confirmed with specific antibody by western blot technique.
Results: The modified M2 gene was expressed in E-coli. The concentration of it was 300?g/ml and it was confirmed by western blotting with specific antibody.
Conclusion: Influenza M2 protein with modification in sequence could be expressed in BL21 E-coli host.

 
Keyword(s): CLONING, INFLUENZA VIRUS, PROTEIN EXPRESSION, M2 PROTEIN, GENETIC MODIFICATION, PET22B
 
References: 
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