Paper Information

Journal:   PAJOUHESH DAR PEZESHKI   2015 , Volume 39 , Number 1; Page(s) 9 To 13.
 
Paper: 

ASSESSING THE GENE EXPRESSION OF IL4, TNFα, TGFβ AND IFNγ IN STUDYING M1 AND M2 MACROPHAGES DERIVED FROM HUMAN MONOCYTE

 
 
Author(s):  JALILI ARSALAN, MOSLEMI ELHAM*, MOSAFFA NARIMAN
 
* DEPARTMENT OF BIOLOGY SCHOOL OF BASIC SCIENCE, ISLAMIC AZAD UNIVERSITY, EAST TEHRAN BRANCH, TEHRAN IRAN
 
Abstract: 

Background: Classical macrophages (M1) and alternative macrophages (M2) are responsible for various functions in order to maintain homeostasis. BCG vaccine and hydatid cyst fluid can be examples of stimulants, which can cause M1 and M2 macrophage polarization. Evaluating the expression of markers such as IFNg and TNFa for M1 phenotype and TGFb and IL4 for M2 phenotype is one of the confirmatory ways of polarization. The purpose of this study is to verify the polarization of macrophages, which was induced by BCG vaccine and hydatid cyst fluid by studying the gene expression of aforementioned markers.
Methods: Using ficol 1077 and gradient concentration separated mononuclear normal human peripheral blood cells. Monocyte populations were separated by adhesion technique from cultured cells in complete tissue culture medium. Monocyte derived macrophages were treated by BCG intravesical vaccine and fertile hydatid cyst fluid for 8 hours. RT-PCR was performed to confirm the polarization. The expression of amplified genes was compared with control groups.
Results: Amplification of TNF
a and IFNg genes in M1, and TGFb and IL4 in M2 group confirmed the polarization procedure. Moreover, lack of PCR product in negative control samples indicates either the absence or a very low expression of this gene in control group.
Conclusion: The ability of these stimulants in macrophage polarization can be used in experimental studies. It is also possible to take advantage of this model to achieve useful goals in cell therapy and develop more efficient therapeutic methods in inflammatory diseases and cancer immunotherapy.

 
Keyword(s): CLASSICAL MACROPHAGES, ALTERNATIVE MACROPHAGES, POLARIZATION, BCG, HYDATID CYST FLUID
 
References: 
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