The paper presents the first contribution to the knowledge of the leaf miner flies (Diptera: Agromyzidae) of the Urmia region in the West Azerbaijan province and a list of the 46 recorded species of agromyzid flies of Iran. During a three-year survey, 20 agromyzid species were identified belonging to nine genera. The species Amauromyza (Cephalomyza) luteiceps (Handel 1920), Aulagromyza populi (Kaltenbach, 1864), Cerodontha (Poemyza) lapplandica Ryden, 1956, Liriomyza hieracii (Kaltenbach, 1862), Melanoagromyza aeneoventris (Falleh, 1823), Melanoagromyza albocilia Handel, 1931, Melanoagromyza nigrissima Spencer, 1976, Ophiomyia beckeri (Hendel, 1923), Ophiomyia curvipalpis (Zerterstedt, 1848), Ophiomyia orbculata (Hendel, 1931) and Pseudonapomyza europaea Spencer, 1973 werereported as new records for the Iranian insect fauna.