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Paper Information

Journal:   GENETICS IN THE 3RD MILLENNIUM   WINTER 2016 , Volume 14 , Number 1; Page(s) 0 To 0.
 
Paper: 

GENE EXPRESSION ALTERATION IN SUICIDE VICTIMS WITH PSYCHOSIS

 
 
Author(s):  HAGHIGHATFARD ARVIN*, ALIZADENIK ATIEH, RASTEGARIMOGHADDAM ELHAM, NEJATI NOSHIN
 
* DEPARTMENT OF BIOLOGY, NORTH TEHRAN BRANCH, ISLAMIC AZAD UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Suicide is a major public health concern, with approximately one million people committing suicide world-wide each year. Risk factors for suicidal behavior include, psychiatric and medical illness, impulsivity, aggression, alcohol and drug abuse specially stimulants, and stress. Neurobiological, brain mappings and biochemical studies have revealed that suicide victims experiencing a large number of changes in metabolic pathways that end to committing suicide. Our study aimed to discover gene expression changes in these subjects.
Blood samples collected from 84 saved suicide victims with at least one attempt to suicide in last 12 months that suffering from psychosis (50 SCZ, 19MDD, 15 BPD) and 70 gender, age, socioeconomic matched subjects with psychosis (40 SCZ, 12MDD, 18BPD) without any suicidal thoughts. After RNA extraction and c DNA synthesis, Gene expression profiling analysis was done using the Affymetrix Gene Chip Human Genome U133 plus 2.0 Array Platform containing probes representing 39, 000 genes. Preparation of labeled and fragmented a RNA targets, hybridization, and scanning were carried out according to the manufacturer' s protocol (Affymetrix Santa Clara, CA).Microarray results confirmed by real time PCR. Gene ontology analysis was conducted for differentially expressed genes (fold change greater than 2 and a P value less than 0.05) using the enrichment algorithm integrated in the online Database for Annotation, Visualization and Integrated Discovery (DAVID 6.7) Results showed significantly over expression of 65 genes including dopamine receptors and mitochondrial complex I and II genes in suicide victims. Also significantly low expression of 21 genes including COMT, NRG1 and GRM3 has been detected in suicide victims. Regulation of dopamine metabolic process and Central Nervous System Development were main terms indicated in enriched gene ontology calculations.
It seems that gene expression changes in neurodevelopmental and dopaminergic pathways are correlated to suicide tendency and this is related to neuorobilogical functions and decision making processes of brain.
Results of this project can help to predict the possibility of committing suicide in psychotic patients and suggesting potential markers for suicide thoughts.

 
Keyword(s): SUICIDE, GENE EXPRESSION, DOPAMINE METABOLIC, CNS, ENRICHED GENE ONTOLOGY
 
 
References: 
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APA: Copy

HAGHIGHATFARD, A., & ALIZADENIK, A., & RASTEGARIMOGHADDAM, E., & NEJATI, N. (2016). GENE EXPRESSION ALTERATION IN SUICIDE VICTIMS WITH PSYCHOSIS. GENETICS IN THE 3RD MILLENNIUM, 14(1), 0-0. https://www.sid.ir/en/journal/ViewPaper.aspx?id=508723



Vancouver: Copy

HAGHIGHATFARD ARVIN, ALIZADENIK ATIEH, RASTEGARIMOGHADDAM ELHAM, NEJATI NOSHIN. GENE EXPRESSION ALTERATION IN SUICIDE VICTIMS WITH PSYCHOSIS. GENETICS IN THE 3RD MILLENNIUM. 2016 [cited 2021May16];14(1):0-0. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508723



IEEE: Copy

HAGHIGHATFARD, A., ALIZADENIK, A., RASTEGARIMOGHADDAM, E., NEJATI, N., 2016. GENE EXPRESSION ALTERATION IN SUICIDE VICTIMS WITH PSYCHOSIS. GENETICS IN THE 3RD MILLENNIUM, [online] 14(1), pp.0-0. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508723.



 
 
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