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Paper Information

Journal:   PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)   FALL 2015 , Volume 18 , Number 3; Page(s) 75 To 85.
 
Paper: 

FREQUENCY AND MOLECULAR IDENTIFICATION OF LEIS HMANIA PARASITES IN SMEARS PREPARED FROM SKIN LESIONS OF PATIENTS REFERRED TO HEALTH CENTERS OF ILAM PROVINCE BY DIGESTION OF THE RDNA-ITS1 GENE

 
 
Author(s):  GHOLAM PARIZAD ESKANDAR, MALEKI RAVASAN NASEH, GHOLAMI PARIZAD ELAHE, KARIMIAN FATEH*, KARIMIAN BEHROOZ
 
* DEPARTMENT OF MEDICAL ENTOMOLOGY AND VECTOR CONTROL, SCHOOL OF PUBLIC HEALTH, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Objective: Ilam is a border province and a high risk zone for zoonotic cutaneous leishmaniasis (ZCL). Identification of Leishmania parasite species in clinical infections is a prerequisite for planning appropriate control measures. This study investigates the demographic characteristics of patients and molecular epidemiology of Leishmania parasites in the skin lesions of patients from Ilam Province.
Methods: A total of 106 cases of suspected cutaneous leishmaniasis were detected passively and microscopic slides prepared from their active skin lesions. We randomly selected 50 slides. A fragment of the rDNA-ITS1 gene was amplified after which the PCR products digested with HaeIII restriction enzyme. There were 18 samples sequenced and their phylogenetic relationships compared with sequences retrieved from GenBank.
Results: Leishmania amastigotes were detected in 100 slides. The highest and lowest distribution of cases was from the Moosian and Dehloran districts, respectively. There were 68.9% males and 31.1% of cases were women. The RFLP pattern of all samples was similar to Leishmania major. Phylogenetic relationships displayed great similarity between our sequences and those of Leishmania major parasites from sandflies trapped in Ilam and South Khorasan Provinces and human hosts from Esfarayen, Mahshahr and Afghanistan plus Leishmania mexicana of Venezuelan origin classified together in the same clade.
Conclusion: Due to homogeneous morphology, problems associated with the cultivation of Leishmania and the two-step molecular identification process, the rDNA-ITS1-RFLP method has gained considerable attention in recent years. This method could be used as a very sensitive, simple, rapid and inexpensive method to detect Leishmania parasites in a variety of clinical and non-clinical samples.

 
Keyword(s): LEISHMANIA MAJOR, RDNA-ITS1, RFLP, ILAM, HIGH RISK
 
 
References: 
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APA: Copy

GHOLAM PARIZAD, E., & MALEKI RAVASAN, N., & GHOLAMI PARIZAD, E., & KARIMIAN, F., & KARIMIAN, B. (2015). FREQUENCY AND MOLECULAR IDENTIFICATION OF LEIS HMANIA PARASITES IN SMEARS PREPARED FROM SKIN LESIONS OF PATIENTS REFERRED TO HEALTH CENTERS OF ILAM PROVINCE BY DIGESTION OF THE RDNA-ITS1 GENE. PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 18(3), 75-85. https://www.sid.ir/en/journal/ViewPaper.aspx?id=508703



Vancouver: Copy

GHOLAM PARIZAD ESKANDAR, MALEKI RAVASAN NASEH, GHOLAMI PARIZAD ELAHE, KARIMIAN FATEH, KARIMIAN BEHROOZ. FREQUENCY AND MOLECULAR IDENTIFICATION OF LEIS HMANIA PARASITES IN SMEARS PREPARED FROM SKIN LESIONS OF PATIENTS REFERRED TO HEALTH CENTERS OF ILAM PROVINCE BY DIGESTION OF THE RDNA-ITS1 GENE. PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES). 2015 [cited 2021May14];18(3):75-85. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508703



IEEE: Copy

GHOLAM PARIZAD, E., MALEKI RAVASAN, N., GHOLAMI PARIZAD, E., KARIMIAN, F., KARIMIAN, B., 2015. FREQUENCY AND MOLECULAR IDENTIFICATION OF LEIS HMANIA PARASITES IN SMEARS PREPARED FROM SKIN LESIONS OF PATIENTS REFERRED TO HEALTH CENTERS OF ILAM PROVINCE BY DIGESTION OF THE RDNA-ITS1 GENE. PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), [online] 18(3), pp.75-85. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508703.



 
 
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