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Paper Information

Journal:   PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)   FALL 2015 , Volume 18 , Number 3; Page(s) 97 To 107.
 
Paper: 

INVESTIGATION OF ERG11 GENE MUTATIONS IN FLUCONAZOLE RESISTANT CANDIDA ALBICANS ISOLATED FROM A NUMBER OF RASHT HOSPITALS

 
 
Author(s):  EFTEKHARI AZADEH DOKHT, ANVARI MASUMEH*, RANJI NAJMEH
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCES, RASHT BRANCH OF ISLAMIC AZAD UNIVERSITY, RASHT, IRAN, P.O.BOX: 41335-3516
 
Abstract: 

Objective: Azole antifungal drugs have been a treatment option for Candida albicans infections. However, azole resistance can occur through different mechanisms such as alterations in ERG11 (lanosterol 14?-demethylase). This study assesses ERG11 gene mutations in Candida albicans strains isolated from patients with Candidia Volvo vaginitis in a number of Rasht hospitals between 2012-2014 by direct PCR and sequencing.
Methods: We identified the yeast strains by standard identification methods, such as germ tubes. Drug sensitivity was determined as MIC 90 values by the macro dilution broth method based on the CLSI protocol. We screened the resistant strains prior to DNA extraction and ERG11 gene mutations were confirmed by PCR sequencing.
Results: From 40 strains, 4 showed high levels of resistance to fluconazole. Of these, two species had a MIC 90 of 512
mg/ml and the other two species had a MIC 90 of 1024 mg/ml. Three strains had D116E and V456G polymorphisms.
Conclusion: The most fluconazole resistant Candida albicans strains worldwide were reported. Our results suggested a correlation between the polymorphism and fluconazole resistance in the Candida albicans strains.

 
Keyword(s): CANDIDA ALBICANS, FLUCONAZOLE, ERG11
 
 
References: 
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Click to Cite.
APA: Copy

EFTEKHARI, A., & ANVARI, M., & RANJI, N. (2015). INVESTIGATION OF ERG11 GENE MUTATIONS IN FLUCONAZOLE RESISTANT CANDIDA ALBICANS ISOLATED FROM A NUMBER OF RASHT HOSPITALS. PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 18(3), 97-107. https://www.sid.ir/en/journal/ViewPaper.aspx?id=508701



Vancouver: Copy

EFTEKHARI AZADEH DOKHT, ANVARI MASUMEH, RANJI NAJMEH. INVESTIGATION OF ERG11 GENE MUTATIONS IN FLUCONAZOLE RESISTANT CANDIDA ALBICANS ISOLATED FROM A NUMBER OF RASHT HOSPITALS. PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES). 2015 [cited 2021May14];18(3):97-107. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508701



IEEE: Copy

EFTEKHARI, A., ANVARI, M., RANJI, N., 2015. INVESTIGATION OF ERG11 GENE MUTATIONS IN FLUCONAZOLE RESISTANT CANDIDA ALBICANS ISOLATED FROM A NUMBER OF RASHT HOSPITALS. PATHOBIOLOGY REAEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), [online] 18(3), pp.97-107. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=508701.



 
 
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